Intergenerational continuity of cell shape dynamics in Caulobacter crescentus
| Autor: | Aaron R. Dinner, Shiladitya Banerjee, Charles S. Wright, Sean Crosson, Srividya Iyer-Biswas, Norbert F. Scherer |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
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media_common.quotation_subject Shape dynamics Asymmetry Article 03 medical and health sciences Cell Behavior (q-bio.CB) Caulobacter crescentus Microscopy Phase-Contrast 030304 developmental biology media_common Physics 0303 health sciences Multidisciplinary biology 030306 microbiology Plane (geometry) Dynamics (mechanics) Division (mathematics) Models Theoretical biology.organism_classification Exponential function FOS: Biological sciences Quantitative Biology - Cell Behavior Biological system Algorithms |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | We investigate the intergenerational shape dynamics of single Caulobacter crescentus cells using a novel combination of imaging techniques and theoretical modeling. We determine the dynamics of cell pole-to-pole lengths, cross-sectional widths and medial curvatures from high accuracy measurements of cell contours. Moreover, these shape parameters are determined for over 250 cells across approximately 10000 total generations, which affords high statistical precision. Our data and model show that constriction is initiated early in the cell cycle and that its dynamics are controlled by the time scale of exponential longitudinal growth. Based on our extensive and detailed growth and contour data, we develop a minimal mechanical model that quantitatively accounts for the cell shape dynamics and suggests that the asymmetric location of the division plane reflects the distinct mechanical properties of the stalked and swarmer poles. Furthermore, we find that the asymmetry in the division plane location is inherited from the previous generation. We interpret these results in terms of the current molecular understanding of shape, growth and division of C. crescentus. |
| Databáze: | OpenAIRE |
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