A sensitive two-photon probe to selectively detect monoamine oxidase B activity in Parkinson's disease models
| Autor: | Biwei Zhu, Qing Zhu, Lin Li, Grace Y. J. Chen, Eng-King Tan, Shao Q. Yao, Chou Chai, Kah-Leong Lim, Qing-Hua Xu, Chengwu Zhang |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Parkinson's disease Monoamine oxidase General Physics and Astronomy Molecular Probe Techniques Endogeny General Biochemistry Genetics and Molecular Biology Parkin medicine Animals Humans Monoamine Oxidase Multidisciplinary Chemistry Parkinson Disease General Chemistry Hep G2 Cells medicine.disease Molecular biology Small molecule Mice Inbred C57BL Disease Models Animal Biochemistry Case-Control Studies Molecular Probes Biomarker (medicine) Drosophila Female Monoamine oxidase B Molecular probe |
| Zdroj: | Nature communications. 5 |
| ISSN: | 2041-1723 |
| Popis: | The unusually high MAO-B activity consistently observed in Parkinson's disease (PD) patients has been proposed as a biomarker; however, this has not been realized due to the lack of probes suitable for MAO-B-specific detection in live cells/tissues. Here we report the first two-photon, small molecule fluorogenic probe (U1) that enables highly sensitive/specific and real-time imaging of endogenous MAO-B activities across biological samples. We also used U1 to confirm the reported inverse relationship between parkin and MAO-B in PD models. With no apparent toxicity, U1 may be used to monitor MAO-B activities in small animals during disease development. In clinical samples, we find elevated MAO-B activities only in B lymphocytes (not in fibroblasts), hinting that MAO-B activity in peripheral blood cells might be an accessible biomarker for rapid detection of PD. Our results provide important starting points for using small molecule imaging techniques to explore MAO-B at the organism level. |
| Databáze: | OpenAIRE |
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