AtCesA8-driven OsSUS3 expression leads to largely enhanced biomass saccharification and lodging resistance by distinctively altering lignocellulose features in rice
| Autor: | Jingyang Li, Xukai Li, Yanting Wang, Lingqiang Wang, Yuanyuan Tu, Tao Xia, Xiwen Cai, Shengqiu Feng, Leiming Wu, Liangcai Peng, Chunfen Fan, Jiangfeng Huang |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Starch lcsh:Biotechnology Biomass Genetically modified crops Management Monitoring Policy and Law 01 natural sciences Applied Microbiology and Biotechnology lcsh:Fuel Cell wall 03 medical and health sciences chemistry.chemical_compound lcsh:TP315-360 lcsh:TP248.13-248.65 Cellulose Cell wall modification Cellulose crystallinity Sucrose synthase Transgenic rice Renewable Energy Sustainability and the Environment Research food and beverages Genetically modified rice Horticulture 030104 developmental biology General Energy chemistry Agronomy Biomass saccharification Lodging resistance Secondary cell wall Yeast fermentation 010606 plant biology & botany Biotechnology |
| Zdroj: | Biotechnology for Biofuels, Vol 10, Iss 1, Pp 1-12 (2017) Biotechnology for Biofuels |
| ISSN: | 1754-6834 |
| DOI: | 10.1186/s13068-017-0911-0 |
| Popis: | Background Biomass recalcitrance and plant lodging are two complex traits that tightly associate with plant cell wall structure and features. Although genetic modification of plant cell walls can potentially reduce recalcitrance for enhancing biomass saccharification, it remains a challenge to maintain a normal growth with enhanced biomass yield and lodging resistance in transgenic plants. Sucrose synthase (SUS) is a key enzyme to regulate carbon partitioning by providing UDP-glucose as substrate for cellulose and other polysaccharide biosynthesis. Although SUS transgenic plants have reportedly exhibited improvement on the cellulose and starch based traits, little is yet reported about SUS impacts on both biomass saccharification and lodging resistance. In this study, we selected the transgenic rice plants that expressed OsSUS3 genes when driven by the AtCesA8 promoter specific for promoting secondary cell wall cellulose synthesis in Arabidopsis. We examined biomass saccharification and lodging resistance in the transgenic plants and detected their cell wall structures and wall polymer features. Results During two-year field experiments, the selected AtCesA8::SUS3 transgenic plants maintained a normal growth with slightly increased biomass yields. The four independent transgenic lines exhibited much higher biomass enzymatic saccharification and bioethanol production under chemical pretreatments at P |
| Databáze: | OpenAIRE |
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