Mapping of antigenic epitopes within the recombinant human preproinsulin related to insulin-dependent diabetes mellitus
| Autor: | Michael Walter, Heike Berg, Wolfgang Northemann |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Microbiology (medical)
Signal peptide Preproinsulin Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Epitope law.invention Epitopes Antigen Affinity chromatography law medicine Humans Insulin Immunology and Allergy Protein Precursors Escherichia coli Autoantibodies Biochemistry (medical) Public Health Environmental and Occupational Health Autoantibody Hematology Molecular biology Recombinant Proteins Medical Laboratory Technology Diabetes Mellitus Type 1 Recombinant DNA Proinsulin |
| Zdroj: | Journal of Clinical Laboratory Analysis. 8:163-166 |
| ISSN: | 1098-2825 0887-8013 |
| DOI: | 10.1002/jcla.1860080309 |
| Popis: | The human insulin domains, signal peptide, B-chain, C-peptide, and A-chain, were highly expressed in Escherichia coli as recombinant proteins N-terminally fused to glutathione-S-transferase and a histidinehexapeptide. The recombinant proteins were purified from insoluble cell fraction by affinity chromatography using metal chelating matrix, which was charged with Ni+2 iones. ELISA screening for autoantibodies directed to preproinsulin were performed with sera from patients with recently diagnosed insulindependent diabetes mellitus in order to localize the antigenic epitopes within the human preproinsulin. Of the patients, 14% had developed autoantibodies that recognized either the recombinant C-peptide or the signal peptide. No reaction was observed with the A-chain or B-chain. © 1994 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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