Emergence of CTX-M-15-producing Klebsiella pneumoniae of multilocus sequence types 1, 11, 14, 17, 20, 35 and 36 as pathogens and colonizers in newborns and adults
| Autor: | Rubén González-Sanz, Inmaculada López-Rodríguez, Verónica Bautista, Juan García-Caballero, Pilar Marín-Casanova, Salvador Oña-Compán, María Pérez-Vázquez, Ana Banderas-Florido, Jesús Oteo, Oscar Cuevas, Silvia García-Cobos, José Campos, Margarita Arroyo, Ana Vindel, Víctor Fuentes-Gómez |
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| Rok vydání: | 2009 |
| Předmět: |
Electrophoresis
Adult Microbiology (medical) Genotype Klebsiella pneumoniae/classification Sequence analysis Klebsiella pneumoniae Drug resistance Biology Polymerase Chain Reaction beta-Lactamases Anti-Bacterial Agents/pharmacology Microbiology Pulsed-Field Plasmid Klebsiella Infections/microbiology Pulsed-field gel electrophoresis Cluster Analysis Humans Pharmacology (medical) beta-Lactamases/biosynthesis Pharmacology Cross Infection Gel Molecular Epidemiology Molecular epidemiology Infant Newborn Infant DNA Sequence Analysis DNA biochemical phenomena metabolism and nutrition Newborn bacterial infections and mycoses biology.organism_classification Hospitals Anti-Bacterial Agents Klebsiella Infections Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Infectious Diseases Spain Carrier State Carrier State/microbiology Multilocus sequence typing Cross Infection/microbiology Sequence Analysis Plasmids |
| Zdroj: | Journal of Antimicrobial Chemotherapy, 64(3), 524-528. Oxford University Press |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dkp211 |
| Popis: | OBJECTIVES: To characterize the population structure and resistance mechanisms of Klebsiella pneumoniae isolates that are highly resistant to third-generation cephalosporins, collected from five Spanish hospitals.METHODS: A total of 162 K. pneumoniae isolates from five hospitals located in three geographical areas of Spain were characterized. The number of isolates from each hospital ranged from 3 to 82. The genetic relationship between isolates was established by PFGE and multilocus sequence typing (MLST). bla(ESBL) types and other antibiotic resistance genes were analysed by PCR and sequencing. Plasmids were classified according to their incompatibility group by a PCR-based replicon-typing scheme.RESULTS: All 162 isolates carried the bla(CTX-15) gene. Fifty-eight isolates (35.8%) caused clinical infections and 104 (64.2%) were colonizers. Sixty-nine (42.6%) isolates were collected from newborns and 93 (57.4%) from adults. Using PGFE, the 162 isolates were grouped into seven clusters that were further identified as members of the MLST types 1, 11, 14, 17, 20, 35 and 36. Two hospitals each had two different clones and the remaining three hospitals had a single CTX-M-15-producing K. pneumoniae clone. All clones carried different antibiotic resistance genes, including bla(OXA-1), aac(3)-IIa, aac(6')-Ib-cr, qnrS1 and qnrB. In four of the seven (57.1%) clones the bla(CTX-M-15) gene was transferred by conjugation; in all cases plasmids of the incompatibility group IncF were identified by PCR.CONCLUSIONS: This study shows that multiresistant K. pneumoniae producing CTX-M-15 of MLST types 1, 11, 14, 17, 20, 35 and 36 are spreading as pathogens and colonizers among newborns and adult patients in Spain. |
| Databáze: | OpenAIRE |
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