Nuclear Localised MORE SULPHUR ACCUMULATION1 Epigenetically Regulates Sulphur Homeostasis in Arabidopsis thaliana
| Autor: | Hermann Bauwe, Brian P. Dilkes, Dai-Yin Chao, Francisco J. Sandoval, Markus Wirtz, David E. Salt, Stanislav Kopriva, Sanja Roje, Xin-Yuan Huang, Steffen Müller, John Danku, Anna Koprivova, Rüdiger Hell |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cancer Research Leaves S-Adenosylmethionine Agricultural Biotechnology Mutant Arabidopsis Artificial Gene Amplification and Extension Plant Science 01 natural sciences Biochemistry Genetically Modified Plants Polymerase Chain Reaction Epigenesis Genetic chemistry.chemical_compound Gene expression Homeostasis Genetics (clinical) 2. Zero hunger DNA methylation Sulfates Plant Anatomy Genetically Modified Organisms Nuclear Proteins Agriculture Methylation Plants Glutathione Chromatin Nucleic acids Chemistry medicine.anatomical_structure Physical Sciences Epigenetics DNA modification Genetic Engineering Chromatin modification Research Article Chromosome biology Chemical Elements Biotechnology Cell biology lcsh:QH426-470 Arabidopsis Thaliana Anion Transport Proteins Active Transport Cell Nucleus Brassica Biology Research and Analysis Methods 03 medical and health sciences Model Organisms Biosynthesis Plant and Algal Models medicine Genetics Molecular Biology Techniques Gene Molecular Biology Ecology Evolution Behavior and Systematics Cell Nucleus Biology and life sciences Arabidopsis Proteins Chemical Compounds Organisms DNA lcsh:Genetics Cell nucleus 030104 developmental biology chemistry 13. Climate action Salts Plant Biotechnology Sulfur 010606 plant biology & botany |
| Zdroj: | PLoS Genetics PLoS Genetics, Vol 12, Iss 9, p e1006298 (2016) |
| ISSN: | 1553-7404 1553-7390 |
| Popis: | Sulphur (S) is an essential element for all living organisms. The uptake, assimilation and metabolism of S in plants are well studied. However, the regulation of S homeostasis remains largely unknown. Here, we report on the identification and characterisation of the more sulphur accumulation1 (msa1-1) mutant. The MSA1 protein is localized to the nucleus and is required for both S-adenosylmethionine (SAM) production and DNA methylation. Loss of function of the nuclear localised MSA1 leads to a reduction in SAM in roots and a strong S-deficiency response even at ample S supply, causing an over-accumulation of sulphate, sulphite, cysteine and glutathione. Supplementation with SAM suppresses this high S phenotype. Furthermore, mutation of MSA1 affects genome-wide DNA methylation, including the methylation of S-deficiency responsive genes. Elevated S accumulation in msa1-1 requires the increased expression of the sulphate transporter genes SULTR1;1 and SULTR1;2 which are also differentially methylated in msa1-1. Our results suggest a novel function for MSA1 in the nucleus in regulating SAM biosynthesis and maintaining S homeostasis epigenetically via DNA methylation. Author Summary Sulphur is an essential element for all living organisms including plants. Plants take up sulphur from the soil mainly in the form of inorganic sulphate. The uptake of sulphate and assimilation of sulphur have been well studied. However, the regulation of sulphur accumulation in plants remains largely unknown. In this study, we characterize the high leaf sulphur mutant more sulphur accumulation1 (msa1-1) and demonstrate the function of MSA1 in controlling sulphur accumulation in Arabidopsis thaliana. The MSA1 protein is localized to the nucleus and is required for the biosynthesis of S-adenosylmethionine (SAM) which is a universal methyl donor for many methylation reactions, including DNA methylation. Loss of function of MSA1 reduces the SAM level in roots and affects genome-wide DNA methylation, including the methylation of sulphate transporter genes. We show that the high sulphur phenotype of msa1-1 requires elevated expression of the sulphate transporter genes which are differentially methylated in msa1-1. Our results suggest a connection between sulphur homeostasis and DNA methylation that is mediated by MSA1. |
| Databáze: | OpenAIRE |
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