Review of Polyclonal Antibody Production Procedures in Mammals and Poultry
| Autor: | James E. Artwohl, B. Taylor Bennett, W. Carey Hanly |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_treatment
General Medicine biochemical phenomena metabolism and nutrition Biology General Biochemistry Genetics and Molecular Biology Immune system Immunization Polyclonal antibodies Humoral immunity Immunology Injection site medicine biology.protein bacteria Production (economics) Animal Science and Zoology Antibody Adjuvant |
| Zdroj: | ILAR journal. 37(3) |
| ISSN: | 1930-6180 |
| Popis: | In recent years the production of antibodies (Abs) in laboratory animals has become an essential part of many research projects. Investigators preparing to produce antibodies are confronted with a number of complex choices, some of which may be critical for success. The goal is to obtain high-titer, high-affinity antisera in a manner consistent with the welfare of the animals being immunized. Investigators not familiar with antibody (Ab) production require some guidelines to carry out immunizations in an appropriate manner. Recent studies of components of the immune system, their functions, and the actions of adjuvants on the immune system have yielded valuable information for understanding immune responses. The following discussion is an attempt to provide the non-immunologist with a rational basis for making decisions about choices of animal, immunization procedures, and adjuvants. An overview of the humoral immune response is included to provide a basis for understanding the function of adjuvants and immunization strategies. Additional information may be obtained from any of several immunology textbooks (Janeway and Travers, 1994; Paul, 1993). Although optimization of procedures is still largely empirical, successful production of useful Abs can usually be accomplished by following currently available guidelines. |
| Databáze: | OpenAIRE |
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