Transcriptional activator, AoXlnR, mediates cellulose-inductive expression of the xylanolytic and cellulolytic genes inAspergillus oryzae
| Autor: | Masashi Kato, Noriyuki Kitamoto, Norihiro Tsukagoshi, Tetsuo Kobayashi, Junichiro Marui |
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| Rok vydání: | 2002 |
| Předmět: |
Aspergillus oryzae
Genes Fungal Biophysics Gene Expression lac operon Biology Biochemistry Microbiology Fungal Proteins Aspergillus oryzae XlnR Cellulase Cellulolytic genes Structural Biology Gene expression Escherichia coli Genetics Northern blot Cellulose Promoter Regions Genetic Molecular Biology Gene Xylanolytic genes Fungal protein Binding Sites Base Sequence Strain (chemistry) food and beverages Cell Biology biology.organism_classification Xylan Endo-1 3-beta-Xylosidase Xylosidases Lac Operon Trans-Activators Xylanase |
| Zdroj: | FEBS Letters. 528:279-282 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(02)03328-8 |
| Popis: | AoXlnR was isolated as a transcriptional activator of the major xylanase gene, xynF1, in Aspergillus oryzae. To investigate the spectrum of genes under the control of AoXlnR, expression of the xylanolytic and cellulolytic genes in an A. oryzae wild type strain, an AoxlnR disruptant and an AoXlnR overexpressed strain was analyzed by Northern blotting. AoXlnR mediated expression of at least four xylanolytic genes and four cellulolytic genes when induced by xylan and D-xylose. Moreover, AoXlnR was newly found to mediate the cellulose-inductive expression of the xylanolytic genes as well as the cellulolytic genes. |
| Databáze: | OpenAIRE |
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