In vitroevaluation of the ability of platelet-rich plasma to seal an iatrogenic fetal membrane defect
| Autor: | Liesbeth Lewi, Marc Hoylaerts, E Poliard, Jan Deprest, Liesbeth Heyns, E. Beutels, Daisy Liekens |
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| Rok vydání: | 2009 |
| Předmět: |
Wound Healing
Amniotic fluid Platelet-Rich Plasma Cell growth Extraembryonic Membranes Obstetrics and Gynecology Biology Matrix (biology) Cell biology Organ Culture Techniques Membrane Cell Movement Cell culture Fetal membrane Platelet-rich plasma embryonic structures Immunology Humans Tissue Adhesives Wound healing Genetics (clinical) |
| Zdroj: | Prenatal Diagnosis. 29:620-625 |
| ISSN: | 1097-0223 0197-3851 |
| DOI: | 10.1002/pd.2249 |
| Popis: | Objectives The purpose of the study is to evaluate the ability of platelet-rich plasma (PRP) to seal an iatrogenic fetal membrane defect. Methods First, we evaluated the stability of a PRP plug in an amniotic fluid environment. Further, we evaluated the sealing capability of PRP plugs in an in vitro model that mimics a fetoscopic membrane defect. Finally, we examined its influence on membrane repair and cell proliferation in monolayer cell cultures and amnion-chorion tissue explants. Results PRP plugs persisted in an amniotic fluid for a median time of 7 weeks. PRP plugs also provided waterproof sealing of a fetoscopic membrane defect. Finally, PRP stimulated cell proliferation in a monolayer cell culture and provided a good matrix for cell proliferation and migration in amnion–chorion tissue explants. Conclusion Our in vitro experiments suggest that PRP plugs may provide a long-lasting, waterproof sealing of fetal membrane defects and stimulate fetal membrane repair. Copyright © 2009 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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