KGF regulates pulmonary epithelial proliferation and surfactant protein gene expression in adult rat lung

Autor: Robin R. Deterding, Tianli Pan, John M. Shannon, Robert J. Mason, Toshiyuki Yano, Larry D. Nielsen
Rok vydání: 2000
Předmět:
Pathology
Physiology
Thyroid Nuclear Factor 1
Gene Expression
chemistry.chemical_compound
Uteroglobin
Growth Substances
In Situ Hybridization
Pulmonary Surfactant-Associated Protein A
Age Factors
Nuclear Proteins
Hyperplasia
Pulmonary Surfactant-Associated Protein D
Specific Pathogen-Free Organisms
medicine.anatomical_structure
Keratinocyte growth factor
Pulmonary alveolus
Bronchoalveolar Lavage Fluid
Cell Division
Bromodeoxyuridine
Pulmonary and Respiratory Medicine
medicine.medical_specialty
Fibroblast Growth Factor 7
Pulmonary Surfactant-Associated Proteins
Proteolipids
Respiratory Mucosa
In situ hybridization
Biology
Physiology (medical)
medicine
Animals
Humans
RNA
Messenger
Glycoproteins
Lung
Cell growth
Proteins
Epithelial Cells
Pulmonary Surfactants
Cell Biology
medicine.disease
Molecular biology
Rats
Inbred F344
Epithelium
Rats
Fibroblast Growth Factors
Pulmonary Alveoli
chemistry
Fibroblast Growth Factor 10
Transcription Factors
Zdroj: American Journal of Physiology-Lung Cellular and Molecular Physiology. 279:L1146-L1158
ISSN: 1522-1504
1040-0605
Popis: Keratinocyte growth factor (KGF, FGF-7) is a potent mitogen for epithelial cells. We instilled recombinant human KGF to determine the effects of KGF on alveolar epithelial cells. Left lungs of adult rats were instilled intrabronchially with KGF (5 mg/kg) or normal saline. KGF instillation resulted in epithelial cell hyperplasia, and the alveolar bromodeoxyuridine (BrdU) labeling index peaked at 35% on day 2 after instillation. The mRNA levels for the surfactant proteins (SPs) SP-A, SP-B, and SP-D were increased in whole lung tissue on days 1 and 2 after KGF treatment and then returned to control levels on days 3–7. SP-C mRNA levels were increased on days 2–5 after KGF instillation. However, all surfactant protein mRNAs were reduced in type II cells isolated from rats instilled with KGF 2 or 3 days before isolation. These observations were confirmed by in situ hybridization. Instillation of KGF also increased the amount of SP-A and SP-D in lavage fluid. Transcripts for CC10, the 10-kDa Clara cell protein, were decreased. KGF increases the mRNA for the surfactant proteins per lung because of type II cell hyperplasia, but the mRNA per cell is slightly diminished as measured in isolated cells or estimated by in situ hybridization.
Databáze: OpenAIRE
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