Enzymatic switching for efficient and accurate translesion DNA replication
| Autor: | Peter M. J. Burgers, Robert J. Kokoska, Thomas A. Kunkel, Carrie M. Welch, Erik Johansson, Scott D. McCulloch, Olga Chilkova |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
DNA Replication
Base Pair Mismatch DNA polymerase DNA polymerase II Pyrimidine dimer DNA-Directed DNA Polymerase Saccharomyces cerevisiae DNA polymerase delta chemistry.chemical_compound Genetics Polymerase DNA Polymerase III Models Genetic biology DNA replication DNA Polymerase II Articles Processivity Exodeoxyribonucleases chemistry Biochemistry Pyrimidine Dimers biology.protein Biophysics DNA DNA Damage |
| Zdroj: | Nucleic Acids Research. 32:4665-4675 |
| ISSN: | 1362-4962 |
| DOI: | 10.1093/nar/gkh777 |
| Popis: | When cyclobutane pyrimidine dimers stall DNA replication by DNA polymerase (Pol) delta or epsilon, a switch occurs to allow translesion synthesis by DNA polymerase eta, followed by another switch that allows normal replication to resume. In the present study, we investigate these switches using Saccharomyces cerevisiae Pol delta, Pol epsilon and Pol eta and a series of matched and mismatched primer templates that mimic each incorporation needed to completely bypass a cis-syn thymine-thymine (TT) dimer. We report a complementary pattern of substrate use indicating that enzymatic switching involving localized translesion synthesis by Pol eta and mismatch excision and polymerization by a major replicative polymerase can account for the efficient and accurate dimer bypass known to suppress sunlight-induced mutagenesis and skin cancer. |
| Databáze: | OpenAIRE |
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