Expression of Cholesterol Sulfotransferase (SULT2B1b) in Human Skin and Primary Cultures of Human Epidermal Keratinocytes
| Autor: | Yuko Higashi, Tomoko Fukushige, Young Mok Lee, Hirotoshi Fuda, Hidekatsu Yanai, Tamotsu Kanzaki, Charles A. Strott |
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| Rok vydání: | 2004 |
| Předmět: |
Keratinocytes
Sulfotransferase keratinocyte Human skin sulfate sulfotransferase Granular layer Dermatology Biology Filaggrin Proteins Isozyme Biochemistry Gene Expression Regulation Enzymologic epidermis medicine Humans Involucrin Molecular Biology Cells Cultured Epidermis (botany) integumentary system Cell Differentiation Cell Biology Molecular biology Immunohistochemistry medicine.anatomical_structure Cholesterol Epidermal Cells Calcium Cholesterol Esters Sulfotransferases Keratinocyte Filaggrin |
| Zdroj: | Journal of Investigative Dermatology. 122(5):1207-1213 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/j.0022-202x.2004.22416.x |
| Popis: | Cholesterol sulfate is a highly amphipathic molecule that is present in a relatively high concentration in the epidermis of human skin, particularly in the granular layer. The physiologic significance of this finding, however, is not well-understood. Therefore, we examined expression of the gene encoding for the enzyme that sulfonates cholesterol (SULT2B1b). Of the three enzymes known to sulfonate steroids/sterols, only the SULT2B1b isozyme was detected in cultures of normal human epidermal keratinocytes (NHEK) in response to Ca 2+ -induced terminal differentiation as well as by normal human epidermal tissue. Immunocytochemical analysis of normal skin as well as specific skin disorders was carried out. In normal skin, the expression of SULT2B1b was localized to the granular layer of the epidermis similar to that of filaggrin, an acknowledged late marker of differentiation and in contrast to that of involucrin, an early marker of terminal differentiation, which was expressed throughout the suprabasal region. The confinement of SULT2B1b to the granular layer coincides with this being the area with the highest cholesterol sulfate content suggesting that the physiologic action of cholesterol sulfate is likely carried out in this region of the living epidermis. Additionally, 88% of cholesterol sulfate in NHEK was membrane-associated further suggesting a cellular location for cholesterol sulfate action. |
| Databáze: | OpenAIRE |
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