Zebrafish as a Model Organism for the Development of Drugs for Skin Cancer

Autor: Reza Changizi, Annalisa Saltari, Elisabetta Palazzo, Mataleena Parikka, Carlo Pincelli, Harlan Barker, Hamed Manouchehri, Ashok Aspatwar, Fatemeh Bootorabi
Přispěvatelé: Lääketieteen ja biotieteiden tiedekunta - Faculty of Medicine and Life Sciences, University of Tampere
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
squamous cell carcinoma
Skin Neoplasms
ved/biology.organism_classification_rank.species
Drug development
Inhibitor screening
Melanoma
Skin cancer
Squamous cell carcinoma
Transgenic zebrafish
Animals
Antineoplastic Agents
Disease Models
Animal
Drug Discovery
Humans
Zebrafish
Review
lcsh:Chemistry
Genome editing
CRISPR
lcsh:QH301-705.5
Spectroscopy
skin cancer
Drug discovery
General Medicine
Computer Science Applications
Computational biology
Biology
Catalysis
Biokemia
solu- ja molekyylibiologia - Biochemistry
cell and molecular biology
Inorganic Chemistry
03 medical and health sciences
medicine
melanoma
Physical and Theoretical Chemistry
transgenic zebrafish
Model organism
Molecular Biology
ved/biology
Animal
Organic Chemistry
inhibitor screening
medicine.disease
biology.organism_classification
drug development
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Immunology
Disease Models
Zdroj: International Journal of Molecular Sciences, Vol 18, Iss 7, p 1550 (2017)
International Journal of Molecular Sciences
ISSN: 1422-0067
Popis: Skin cancer, which includes melanoma and squamous cell carcinoma, represents the most common type of cutaneous malignancy worldwide, and its incidence is expected to rise in the near future. This condition derives from acquired genetic dysregulation of signaling pathways involved in the proliferation and apoptosis of skin cells. The development of animal models has allowed a better understanding of these pathomechanisms, with the possibility of carrying out toxicological screening and drug development. In particular, the zebrafish (Danio rerio) has been established as one of the most important model organisms for cancer research. This model is particularly suitable for live cell imaging and high-throughput drug screening in a large-scale fashion. Thanks to the recent advances in genome editing, such as the clustered regularly-interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) methodologies, the mechanisms associated with cancer development and progression, as well as drug resistance can be investigated and comprehended. With these unique tools, the zebrafish represents a powerful platform for skin cancer research in the development of target therapies. Here, we will review the advantages of using the zebrafish model for drug discovery and toxicological and phenotypical screening. We will focus in detail on the most recent progress in the field of zebrafish model generation for the study of melanoma and squamous cell carcinoma (SCC), including cancer cell injection and transgenic animal development. Moreover, we will report the latest compounds and small molecules under investigation in melanoma zebrafish models.
Databáze: OpenAIRE
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