LINC00922 Accelerates the Proliferation, Migration and Invasion of Lung Cancer Via the miRNA-204/CXCR4 Axis
| Autor: | Bin Wang, Jei Li, Tao Liang, Yang Liu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Receptors CXCR4 China Lung Neoplasms Vimentin 030204 cardiovascular system & hematology Biology CXCR4 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Clinical Research Cell Movement Carcinoma Non-Small-Cell Lung Cell Line Tumor Databases Genetic microRNA medicine Humans Neoplasm Invasiveness Lung cancer Aged Cell Proliferation Regulation of gene expression A549 cell General Medicine Middle Aged respiratory system Cadherins medicine.disease respiratory tract diseases Gene Expression Regulation Neoplastic MicroRNAs Real-time polymerase chain reaction A549 Cells 030220 oncology & carcinogenesis biology.protein Cancer research RNA Long Noncoding Female Signal Transduction |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | BACKGROUND The aim of this study was to clarify the potential function of LINC00922 in regulating the progression of lung cancer and its underling mechanism. MATERIAL AND METHODS Relative levels of LINC00922 in lung cancer tissues and cell lines was determined by quantitative polymerase chain reaction. Correlation between LINC00922 levels and pathological indexes of lung cancer patients was analyzed through the chi-square test. Subsequently, regulatory effects of LINC00922 on the proliferative, migratory, and invasive capacities of PC9 and A549 cells were evaluated. Western blot was conducted to determine the role of LINC00922 in mediating protein levels of CXCR4, E-cadherin, and vimentin. Through dual-luciferase reporter gene assay and functional experiments, the potential function of LINC00922/miRNA-204/CXCR4 regulatory loop in mediating the progression of lung cancer was explored. RESULTS LINC00922 was highly expressed in lung cancer and correlated to the poor prognosis of lung cancer patients. Overexpression of LINC00922 accelerated PC9 and A549 cells to proliferate, migrate, and invade. CXCR4 was upregulated in lung cancer tissues and cells, which promoted lung cancer cells to migrate and invade. LINC00922 regulated the level of CXCR4 and directly bound to miRNA-204/CXCR4. LINC00922 mediated the cellular behaviors of lung cancer cells via targeting the miRNA-204/CXCR4 axis. CONCLUSIONS LINC00922 was upregulated in lung cancer, and accelerated lung cancer cells to proliferate, migrate, and invade via targeting the miRNA-204/CXCR4 axis. |
| Databáze: | OpenAIRE |
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