Evaluation of Hemagglutination Activity of Chitosan Nanoparticles Using Human Erythrocytes
| Autor: | Luiz Carlos Alves, Ronaldo Rodrigues Sarmento, Jefferson Muniz de Lima, Joelma Rodrigues de Souza, Juliano Elvis de Oliveira, Rafael José Ribeiro Padilha, Roberta Ferreti Bonan Dantas Batista, Isaque Jerônimo Porto, Ana Paula Sampaio Feitosa, Paulo Rogério Ferreti Bonan, Fábio André Brayner, Lúcio Roberto Cançado Castellano, Eliton S. Medeiros |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Erythrocytes
Lysis Article Subject Hemagglutination lcsh:Medicine Biocompatible Materials Chitin macromolecular substances Polysaccharide Hemolysis General Biochemistry Genetics and Molecular Biology Neutralization Acetylglucosamine Chitosan chemistry.chemical_compound medicine Humans Particle Size chemistry.chemical_classification General Immunology and Microbiology lcsh:R General Medicine Hydrogen-Ion Concentration medicine.disease Solutions carbohydrates (lipids) Biochemistry chemistry Distilled water Nanoparticles Research Article Nuclear chemistry |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2015 (2015) |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2015/247965 |
| Popis: | Chitosan is a polysaccharide composed of randomly distributed chains ofβ-(1-4) D-glucosamine and N-acetyl-D-glucosamine. This compound is obtained by partial or total deacetylation of chitin in acidic solution. The chitosan-based hemostatic agents have been gaining much attention in the management of bleeding. The aim of this study was to evaluate in vitro hemagglutination activity of chitosan nanoparticles using human erythrocytes. The preparation of nanoparticles was achieved by ionotropic gelification technique followed by neutralization with NaOH 1 mol/L−1. The hemagglutination activity was performed on a solution of 2% erythrocytes (pH 7.4 on PBS) collected from five healthy volunteers. The hemolysis determination was made by spectrophotometric analysis. Chitosan nanoparticle solutions without NaOH addition changed the reddish colour of the wells into brown, suggesting an oxidative reaction of hemoglobin and possible cell lysis. All neutralized solutions of chitosan nanoparticles presented positive haemagglutination, without any change in reaction color. Chitosan nanoparticles presented hemolytic activity ranging from 186.20 to 223.12%, while neutralized solutions ranged from 2.56 to 72.54%, comparing to distilled water. Results highlight the need for development of new routes of synthesis of chitosan nanoparticles within human physiologic pH. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|