Characterization of 29 polymorphic microsatellite markers developed by genomic screening of Sumatran rhinoceros (Dicerorhinus sumatrensis)
| Autor: | Alfred L. Roca, Widodo Ramono, Helena Suryadi, Sinta H. Saidah, Oliver A. Ryder, Peter J. Van Coeverden de Groot, Herawati Sudoyo, Kai Zhao, Isabella Apriyana, Jessica R. Brandt, Yasuko Ishida |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:Medicine Rhinoceros 010603 evolutionary biology 01 natural sciences General Biochemistry Genetics and Molecular Biology Genomic screening 03 medical and health sciences Critically endangered Animals Polymorphic Microsatellite Marker lcsh:Science (General) Genotyping lcsh:QH301-705.5 Perissodactyla Genome biology lcsh:R Genomics General Medicine Amplicon Dicerorhinus sumatrensis biology.organism_classification Research Note Non-invasive sampling 030104 developmental biology Short tandem repeats lcsh:Biology (General) Indonesia Evolutionary biology Microsatellite Microsatellite Repeats lcsh:Q1-390 |
| Zdroj: | BMC Research Notes, Vol 14, Iss 1, Pp 1-7 (2021) BMC Research Notes |
| ISSN: | 1756-0500 |
| Popis: | Objective The Sumatran rhinoceros is critically endangered, with fewer than 100 individuals surviving across its current range. Accurate census estimates of the remaining populations are essential for development and implementation of conservation plans. In order to enable molecular censusing, we here develop microsatellite markers with amplicon sizes of short length, appropriate for non-invasive fecal sampling. Results Due to limited sample quantity and potential lack of genome-wide diversity, Illumina sequence reads were generated from two Sumatran rhinoceros samples. Genomic screening identified reads with short tandem repeats and loci that were polymorphic within the dataset. Twenty-nine novel polymorphic microsatellite markers were characterized (A = 2.4; HO = 0.30). These were sufficient to distinguish among individuals (PID PID(sib) |
| Databáze: | OpenAIRE |
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