Recognition and Unfolding of c-MYC and Telomeric G-Quadruplex DNAs by the RecQ C-Terminal Domain of Human Bloom Syndrome Helicase
| Autor: | Suyeong Han, Chin-Ju Park, Jinwoo Kim, Sungjin Lee |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
biology
General Chemical Engineering C-terminus Hoogsteen base pair Helicase General Chemistry G-quadruplex medicine.disease Article Nucleic acid secondary structure Cell biology DNA metabolism Chemistry chemistry.chemical_compound chemistry medicine biology.protein heterocyclic compounds Bloom syndrome QD1-999 DNA |
| Zdroj: | ACS Omega, Vol 5, Iss 24, Pp 14513-14522 (2020) ACS Omega |
| ISSN: | 2470-1343 |
| DOI: | 10.1021/acsomega.0c01176 |
| Popis: | G-quadruplex (G4) is a noncanonical DNA secondary structure formed by Hoogsteen base pairing. It is recognized by various DNA helicases involved in DNA metabolism processes such as replication and transcription. Human Bloom syndrome protein (BLM), one of five human RecQ helicases, is a G4 helicase. While several studies revealed the mechanism of G4 binding and unfolding by the conserved RecQ C-terminal (RQC) domain of BLM, how RQC recognizes different G4 topologies is still unclear. Here, we investigated the interaction of Myc-22(14/23T) G4 from the c-Myc promoter and hTelo G4 from the telomeric sequence with RQC. Myc-22(14/23T) and hTelo form parallel and (3+1) hybrid topologies, respectively. Our circular dichroism (CD) spectroscopy data indicate that RQC can partially unfold the parallel G4, even with a short 3′ overhang, while it can only partially unfold the (3+1) hybrid G4 with a 3′ overhang of 6 nucleotides or longer. We found that the intrinsic thermal stability of G4 does not determine RQC-induced G4 unfolding by comparing Tm of G4s. We also showed that both parallel and (3+1) hybrid G4s bind to the β-wing region of RQC. Thermodynamic analysis using isothermal titration calorimetry (ITC) showed that all interactions were endothermic and entropically driven. We suggest that RQC partially unfolds the parallel G4 more efficiently than the (3+1) hybrid G4 and binds to various G4 structures using its β-wing region. By this information, our research provides new insights into the influence of G4 structure on DNA metabolic processes involving BLM. |
| Databáze: | OpenAIRE |
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