Development and Validation of a PulseNet Standardized Protocol for Subtyping Isolates ofCronobacterSpecies
| Autor: | Séamus Fanning, Nicole Cernela, Efrain M. Ribot, Franco Pagotto, Stephen B. O'Brien, Carol Iversen, Norma Binsztein, Kara Cooper, Bette Jensen, Roger Stephan, Mariana Pichel, Matthew J. Arduino, Silvina P. Brengi |
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| Rok vydání: | 2012 |
| Předmět: |
DNA
Bacterial Yersinia pestis medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Foodborne Diseases Cronobacter sakazakii Food Preserved Pulsed-field gel electrophoresis medicine Animals Humans Shigella Amplified Fragment Length Polymorphism Analysis Cronobacter Vibrio cholerae biology Enterobacteriaceae Infections Pulsenet Reproducibility of Results DNA Restriction Enzymes Enterobacter biology.organism_classification Virology Subtyping Electrophoresis Gel Pulsed-Field Molecular Typing Restriction enzyme Milk Animal Science and Zoology Food Science |
| Zdroj: | Foodborne Pathogens and Disease. 9:861-867 |
| ISSN: | 1556-7125 1535-3141 |
| Popis: | Cronobacter (formerly known as Enterobacter sakazakii) is a genus comprising seven species regarded as opportunistic pathogens that can be found in a wide variety of environments and foods, including powdered infant formula (PIF). Cronobacter sakazakii, the major species of this genus, has been epidemiologically linked to cases of bacteremia, meningitis in neonates, and necrotizing enterocolitis, and contaminated PIF has been identified as an important source of infection. Robust and reproducible subtyping methods are required to aid in the detection and investigation, of foodborne outbreaks. In this study, a pulsed-field gel electrophoresis (PFGE) protocol was developed and validated for subtyping Cronobacter species. It was derived from an existing modified PulseNet protocol, wherein XbaI and SpeI were the primary and secondary restriction enzymes used, generating an average of 14.7 and 20.3 bands, respectively. The PFGE method developed was both reproducible and discriminatory for subtyping Cronobacter species. |
| Databáze: | OpenAIRE |
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