Role of shiga-like toxin I in bacterial enteritis: Comparison between isogenic Escherichia coli strains induced in rabbits
| Autor: | Joseph Fondacaro, Edgar C. Boedeker, John W. Newland, P. Gemski, Robert Sjogren, David Fritz, Roger Neill, Curtis A. Colleton, Daniel Rachmilewitz, Douglas D. Sharpnack |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Bacterial Toxins Shiga Toxin 1 medicine.disease_cause Virulence factor Enteritis Microbiology Enterotoxins Escherichia coli medicine Animals Humans Shigella Platelet Activating Factor Colitis Pathogen Hepatology biology Enterocolitis Toxin Gastroenterology medicine.disease biology.organism_classification Virology Enterobacteriaceae Rabbits HeLa Cells Interleukin-1 |
| Zdroj: | Gastroenterology. 106:306-317 |
| ISSN: | 0016-5085 |
| Popis: | Background/Aims: Enteroadherent Escherichia coli that produce Shiga-like toxins are important causes of human disease, including enterohemorrhagic E. coli -induced colitis (EHEC). The role of Shiga-like toxins in these illnesses is unclear. The aim of this study was to establish an animal model for human EHEC and to determine the role of Shiga-like toxin I (SLT-I) in this model. Methods: E. coli strain RDEC-1 is an enteroadherent rabbit diarrheal pathogen. An isogenic variant of RDEC-1 (termed RDEC-H19A) producing high levels of SLT-I was obtained by infecting RDEC-1 with an SLT-I-converting bacteriophage. The effects of in vivo enteric infection produced in rabbits by RDEC-H19A were compared with those in uninfected and RDEC-1-in-fected animals. Results: SLT-I-producing RDEC-H19A induced a severe, noninvasive, enteroadherent infection in rabbits. Clinically, infection with RDEC-H19A was more severe than infection with RDEC-1 and caused more serious histological lesions including vascular changes, edema, and more severe inflammation. Interleukin 1 and platelet-activating factor appear to be important inflammatory mediators to this infection. Conclusions: The illness induced by RDEC-H19A in rabbits resembled enterohemorrhagic E. coli -induced colitis of humans. SLT-I is an important virulence factor in the pathogenesis of EHEC. |
| Databáze: | OpenAIRE |
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