Interleukin-1 beta and reactive oxygen species mediate activation of c-Jun NH2-terminal kinases, in human epithelial cells, by two independent pathways
| Autor: | M. Luisa Roberts, Lex M. Cowsert |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
RHOA
Lung Neoplasms Biophysics Small G Protein Arachidonic Acids Biochemistry Wortmannin chemistry.chemical_compound Phosphatidylinositol 3-Kinases Phospholipase A2 GTP-Binding Proteins Tumor Cells Cultured Humans Phosphatidylinositol RNA Messenger Enzyme Inhibitors Molecular Biology Cells Cultured A549 cell biology Chemistry Kinase c-jun Carcinoma JNK Mitogen-Activated Protein Kinases Epithelial Cells Cell Biology Hydrogen Peroxide Oligonucleotides Antisense Molecular biology Cell biology Androstadienes Enzyme Activation Calcium-Calmodulin-Dependent Protein Kinases biology.protein Mitogen-Activated Protein Kinases Reactive Oxygen Species rhoA GTP-Binding Protein Interleukin-1 Signal Transduction |
| Zdroj: | Biochemical and biophysical research communications. 251(1) |
| ISSN: | 0006-291X |
| Popis: | The c-Jun N terminal kinases (JNKs) are members of the mitogen activated protein kinases family, which have been shown to be preferentially activated either by cytokines or stress stimuli. In this study we identify a selective and potent antisense oligonucleotide to RhoA (ISIS 17131) and investigate its effect on JNK activation induced by IL-1beta and H2O2 in A549 cells. The RhoA antisense oligonucleotide was able to inhibit JNK activation when A549 cells were stimulated by H2O2, but did not have any effect on IL-1beta induced JNK activation. Consistent with the idea that the phosphatidylinositol 3-kinase (PI 3-kinase) activates the small G protein exchange factors, H2O2 activated the PI 3-kinase. Additionally, Wortmannin, a potent inhibitor of the PI 3-kinase and phospholipase A2 (PLA2), and AACOCF3, also a PLA2 inhibitor, were able to inhibit JNK activation induced by H2O2, but they had no effect on JNK activation when stimulated by IL-1beta. These results suggest that, in A549, IL-1beta and H2O2 induce JNK activation by two independent pathways. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect