Chronic Two-Photon Calcium Imaging in the Visual Cortex of Awake Behaving Mice
| Autor: | Evelyn Dylda, Janelle M.P. Pakan, Nathalie L. Rochefort |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
chemistry.chemical_element Biology Calcium 03 medical and health sciences Activity monitoring 030104 developmental biology 0302 clinical medicine Visual cortex medicine.anatomical_structure Calcium imaging Two-photon excitation microscopy Neuronal circuits chemistry Cortex (anatomy) medicine Image acquisition ddc:500 Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Amsterdam [u.a.] : Elsevier, Handbook of Behavioral Neuroscience 28, 235-251 (2018). doi:10.1016/B978-0-12-812028-6.00013-6 Handbook of in Vivo Neural Plasticity Techniques / Dylda, Evelyn ; : Elsevier, 2018, ; ISSN: 15697339 ; ISBN: 9780128120286 ; doi:10.1016/B978-0-12-812028-6.00013-6 |
| Popis: | Our ability to learn relies on the potential of neuronal circuits to change through experience. Recent advances in genetic tools and in vivo imaging have enabled novel investigations into the mechanisms of plasticity at the level of neuronal circuits. This chapter describes an experimental method for long-term activity monitoring of large neuronal populations, activity, with single-cell resolution, in the cortex of awake behaving mice. By combining genetically encoded calcium indicators with two-photon imaging, this technique allows researchers to relate the activity of subtypes of neurons directly to the animal's behavior over temporal scales from hundreds of milliseconds to several weeks. We detail the key experimental aspects of long-term, repeated two-photon calcium imaging: surgical procedures for chronic craniotomies, repeated image acquisition, and critical analysis parameters for two-photon calcium imaging data. Finally, we review recent applications of this technique to study the plasticity of neuronal circuits in the primary visual cortex. |
| Databáze: | OpenAIRE |
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