Epithelial-to-Mesenchymal Transition Enhances Cancer Cell Sensitivity to Cytotoxic Effects of Cold Atmospheric Plasmas in Breast and Bladder Cancer Systems
| Autor: | Kostya Ostrikov, Erik W. Thompson, Xiaofeng Dai, Patrick Thomas, Mohit Kumar Jolly, Susmita Mandal, Derek J. Richard, Peiyu Wang, Rusen Zhou, Renwu Zhou, Liqian Zhao, Elizabeth D. Williams, Bernd H. A. Rehm |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Bladder cancer business.industry Mesenchymal stem cell cold atmospheric plasma (CAP) Cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease reactive oxygen species (ROS) Article plasma-activated medium (PAM) Oncology Cell culture Cancer cell embryonic structures medicine Cancer research Carcinoma Cytotoxic T cell Epithelial–mesenchymal transition business epithelial–mesenchymal transition (EMT) RC254-282 |
| Zdroj: | Cancers, Vol 13, Iss 2889, p 2889 (2021) Cancers Volume 13 Issue 12 |
| ISSN: | 2072-6694 |
| Popis: | Cold atmospheric plasma (CAP) has emerged as a highly selective anticancer agent, most recently in the form of plasma-activated medium (PAM). Since epithelial–mesenchymal transition (EMT) has been implicated in resistance to various cancer therapies, we assessed whether EMT status is associated with PAM response. Mesenchymal breast cancer cell lines, as well as the mesenchymal variant in an isogenic EMT/MET human breast cancer cell system (PMC42-ET/LA), were more sensitive to PAM treatment than their epithelial counterparts, contrary to their responses to other therapies. The same trend was seen in luminal muscle-invasive bladder cancer model (TSU-Pr1/B1/B2) and the non-muscle-invasive basal 5637 bladder cancer cell line. Three-dimensional spheroid cultures of the bladder cancer cell lines were less sensitive to the PAM treatment compared to their two-dimensional counterparts however, incrementally better responses were again seen in more mesenchymally-shifted cell lines. This study provides evidence that PAM preferentially inhibits mesenchymally-shifted carcinoma cells, which have been associated with resistance to other therapies. Thus, PAM may represent a novel treatment that can selectively inhibit triple-negative breast cancers and a subset of aggressive bladder cancers, which tend to be more mesenchymal. Our approach may potentially be utilized for other aggressive cancers exhibiting EMT and opens new opportunities for CAP and PAM as a promising new onco-therapy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|