Reciprocal Regulation of Adipocyte and Osteoblast Differentiation of Mesenchymal Stem Cells byEupatorium japonicumPrevents Bone Loss and Adiposity Increase in Osteoporotic Rats
| Autor: | Min-Ji Kim, Woo-Seok Jang, Cho-Rong Seo, Jong-Keun Kim, Young Min Lee, Haeng Ran Kim, No-Joon Song, In-Kyoung Lee, Ki-Moon Park, Min-Hyuk Bang, Kye Won Park, Ki-Seung Seong |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Coumaric Acids Eupatorium Cellular differentiation Medicine (miscellaneous) Eupatorium japonicum Bone Marrow Cells Biology Rats Sprague-Dawley Mice chemistry.chemical_compound Bone Density Internal medicine Adipocyte Adipocytes medicine Animals Adiposity Bone mineral Adipogenesis Osteoblasts Nutrition and Dietetics Plant Stems Plant Extracts Cell Differentiation Mesenchymal Stem Cells Osteoblast 3T3 Cells Alkaline Phosphatase Lipid Metabolism biology.organism_classification Rats Bone Diseases Metabolic Endocrinology medicine.anatomical_structure Primary bone chemistry Osteoporosis Alkaline phosphatase Female Bone marrow Full Communications |
| Zdroj: | Journal of Medicinal Food. 17:772-781 |
| ISSN: | 1557-7600 1096-620X |
| DOI: | 10.1089/jmf.2013.3056 |
| Popis: | Pathological increases in adipogenic potential with decreases in osteogenic differentiation occur in osteoporotic bone marrow cells. Previous studies have shown that bioactive materials isolated from natural products can reciprocally regulate adipogenic and osteogenic fates of bone marrow cells. In this study, we showed that Eupatorium japonicum stem extracts (EJE) suppressed lipid accumulation and inhibited the expression of adipocyte markers in multipotent C3H10T1/2 and primary bone marrow cells. Conversely, EJE stimulated alkaline phosphatase activity and induced the expression of osteoblast markers in C3H10T1/2 and primary bone marrow cells. Daily oral administration of 50 mg/kg of EJE for 6 weeks to ovariectomized rats prevented body weight increase and bone mineral density decrease. Finally, activity-guided fractionation led to the identification of coumaric acid and coumaric acid methyl ester as bioactive anti-adipogenic and pro-osteogenic components in EJE. Taken together, our data indicate a promising possibility of E. japonicum as a functional food and as a therapeutic intervention for preventing osteoporosis and bone fractures. |
| Databáze: | OpenAIRE |
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