SPECIATION ANALYSIS OF ARSENIC IN RICE USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY COUPLED WITH HYDRIDE GENERATION ATOMIC FLUORESCENCE SPECTROMETRY (HPLC-HG-AFS)
| Autor: | Virginia S. T. Ciminelli, Gabriella Alexandre Borges, Guilhermina de O. Souza, Claudia L. Caldeira, Patrícia S. F. Lopes, Guilherme Dias Rodrigues |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Chromatography
Resolution (mass spectrometry) Hydride media_common.quotation_subject rice arsenic Dimethylarsinic Acid chemistry.chemical_element speciation analysis General Chemistry High-performance liquid chromatography Atomic fluorescence spectrometry Chemistry Speciation Certified reference materials chemistry QD1-999 Arsenic media_common |
| Zdroj: | Química Nova, Volume: 43, Issue: 6, Pages: 697-704, Published: 22 JUL 2020 Química Nova v.43 n.6 2020 Química Nova Sociedade Brasileira de Química (SBQ) instacron:SBQ Química Nova, Vol 43, Iss 6, Pp 697-704 (2020) |
| Popis: | The aim of this work was to develop a method using high performance liquid chromatography coupled with hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS) for the determination and speciation analysis of the inorganic and organic species of arsenic found most often in rice samples: As(V), As(III), monomethylarsonic acid (MMA), and dimethylarsinic acid (DMA). The best chromatographic resolution was obtained using pH 6.2, a phosphate buffer mobile phase concentration of 20 mmol L-1 and flow rate of 0.57 mL min-1, HCl concentration of 5.55% (v/v), and NaBH4 concentration of 0.90% (w/v). The accuracy of the method was confirmed by the low relative error values obtained for analysis of a certified reference material (NIST 1568b): 6.63% (total inorganic As), 3.44% (DMA), 0% (MMA), and 0.53% (total As). In recovery tests, the proposed method achieved satisfactory recoveries in the range 86-110%. |
| Databáze: | OpenAIRE |
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