Ebola Virus and Marburg Virus in Human Milk Are Inactivated by Holder Pasteurization
| Autor: | Amy Vickers, Slobodan Paessler, Nadezda Yun, Erin Hamilton Spence, Karen E. Shattuck, Monica Huff |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Viral Plaque Assay Adult Pasteurization medicine.disease_cause Virus law.invention Marburg virus 03 medical and health sciences law medicine Humans Serologic Tests Holder pasteurization Ebola virus and Marburg virus Ebola virus Milk Human business.industry Obstetrics and Gynecology Infant nutrition medicine.disease Ebolavirus Virology Texas 030104 developmental biology Breast Feeding Marburgvirus Milk Banks Female business |
| Zdroj: | Journal of human lactation : official journal of International Lactation Consultant Association. 33(2) |
| ISSN: | 1552-5732 |
| Popis: | Background: Potential donors of human milk are screened for Ebola virus (EBOV) using standard questions, but testing for EBOV and Marburg virus (MARV) is not part of routine serological testing performed by milk banks. Research aim: This study tested the hypothesis that EBOV would be inactivated in donor human milk (DHM) by standard pasteurization techniques (Holder) used in all North American nonprofit milk banks. Methods: Milk samples were obtained from a nonprofit milk bank. They were inoculated with EBOV (Zaire strain) and MARV (Angola strain) and processed by standard Holder pasteurization technique. Plaque assays for EBOV and MARV were performed to detect the presence of virus after pasteurization. Results: Neither EBOV nor MARV was detectable by viral plaque assay in DHM or culture media samples, which were pasteurized by the Holder process. Conclusion: EBOV and MARV are safely inactivated in human milk by standard Holder pasteurization technique. Screening for EBOV or MARV beyond questionnaire and self-deferral is not needed to ensure safety of DHM for high-risk infants. |
| Databáze: | OpenAIRE |
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