Identification of Acinetobacter isolates in the A. calcoaceticus-A. baumannii complex by restriction analysis of the 16S-23S rRNA intergenic-spacer sequences
| Autor: | L. Prandin, Cristina Lagatolla, Enrico Angelo Tonin, Lucilla Dolzani, C. Monti-Bragadin |
|---|---|
| Přispěvatelé: | Dolzani, Lucilla, Tonin, ENRICO ANGELO, Lagatolla, Cristina, Prandin, L., MONTI BRAGADIN, Carlo |
| Rok vydání: | 1995 |
| Předmět: |
Microbiology (medical)
Molecular Sequence Data Biology Microbiology Ribotyping 23S ribosomal RNA RNA Ribosomal 16S Humans Acinetobacter calcoaceticus DNA Primers Genetics Acinetobacter Base Sequence Gene Amplification Spacer DNA Ribosomal RNA biology.organism_classification RNA Bacterial RNA Ribosomal 23S Restriction enzyme bacteria Restriction fragment length polymorphism Genome Bacterial Polymorphism Restriction Fragment Length Research Article |
| Zdroj: | Journal of Clinical Microbiology. 33:1108-1113 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.33.5.1108-1113.1995 |
| Popis: | Members of the genus Acinetobacter are reported to be involved in hospital-acquired infections with an increasing frequency. However, clinical laboratories still lack simple methods that allow complete identification of some pathogenic species, i.e., those corresponding to A. baumannii (DNA group or genospecies 2), unnamed genospecies 3 and 13, and two new genospecies that have recently been described. In fact, a complete discrimination between these species is possible only by DNA-DNA hybridization or ribotyping. Both of these techniques are complex and time-consuming and cannot be performed in most clinical laboratories. As a consequence, isolates belonging to these genospecies are often not differentiated and included, together with the environmental genospecies 1, in the A. calcoaceticus-A. baumannii complex. In this report, a simple and rapid method for the identification of the genospecies belonging to the A. calcoaceticus-A. baumannii complex is proposed. It is based on the combined digestion by the restriction endonuclease AluI and NdeII of the DNA fragments resulting from the amplification of the 16S-23S rRNA intergenic spacer sequences. The analysis of 36 strains characterized by DNA-DNA hybridization in previous studies showed that the restriction profiles obtained are highly reproducible and characteristic for each genospecies. Moreover, extending this study to 68 clinical strains, which were assigned to the A. calcoaceticus-A. baumannii complex by phenotypic tests, confirmed the existence of a panel of limited and well-conserved restriction patterns and allowed the identification of the strains tested. This study thus proposes the detection of restriction length polymorphism in the spacer sequences between the 16S and 23S rRNA genes as a method for the identification of isolates in the A. calcoaceticus-A. baumannii complex. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|