Nucleoporin TPR is an integral component of the TREX-2 mRNA export pathway
| Autor: | Carlos Echeverria, Prasanna Bhat, Alexandra Smith, Hangnoh Lee, Ross Kaufhold, Vasilisa Aksenova, Beatriz M. A. Fontoura, James R. Iben, Mary Dasso, Ka Chun Yau, Shane Chen, Alexei Arnaoutov, Caroline Esnault |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Nucleocytoplasmic Transport Proteins animal structures Science General Physics and Astronomy RNA-binding protein Article Nuclear envelope General Biochemistry Genetics and Molecular Biology Cell Line NXF1 03 medical and health sciences 0302 clinical medicine Gene expression Humans RNA Messenger Nuclear pore lcsh:Science Regulation of gene expression Multidisciplinary Indoleacetic Acids Chemistry Nuclear Proteins RNA-Binding Proteins Zinc Fingers General Chemistry Phosphoproteins Cell biology Nuclear Pore Complex Proteins body regions Protein Transport Exodeoxyribonucleases 030104 developmental biology Gene Expression Regulation nervous system embryonic structures Nuclear Pore lcsh:Q Nucleoporin Degron RNA transport Transcriptome 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-18266-2 |
| Popis: | Nuclear pore complexes (NPCs) are important for cellular functions beyond nucleocytoplasmic trafficking, including genome organization and gene expression. This multi-faceted nature and the slow turnover of NPC components complicates investigations of how individual nucleoporins act in these diverse processes. To address this question, we apply an Auxin-Induced Degron (AID) system to distinguish roles of basket nucleoporins NUP153, NUP50 and TPR. Acute depletion of TPR causes rapid and pronounced changes in transcriptomic profiles. These changes are dissimilar to shifts observed after loss of NUP153 or NUP50, but closely related to changes caused by depletion of mRNA export receptor NXF1 or the GANP subunit of the TRanscription-EXport-2 (TREX-2) mRNA export complex. Moreover, TPR depletion disrupts association of TREX-2 subunits (GANP, PCID2, ENY2) to NPCs and results in abnormal RNA transcription and export. Our findings demonstrate a unique and pivotal role of TPR in gene expression through TREX-2- and/or NXF1-dependent mRNA turnover. mRNAs export from the nucleus is thought to be regulated in part by three nucleoporins that comprise the nuclear basket, but whether and how distinct basket nucleoporins interact with the RNA export machinery is unclear. Here, the authors use rapid auxin-mediated degradation of basket nucleoporins Nup153, Nup50, and Tpr, and see that Tpr interacts with the TREX-2 mRNA export complex. |
| Databáze: | OpenAIRE |
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