Versatile thiol-based reactions for micrometer- and nanometer-scale photopatterning of polymers and biomolecules
| Autor: | Osama El Zubir, C. Neil Hunter, Simone Radl, Matthias Edler, Graham J. Leggett, Robert E. Ducker, Sijing Xia, Michaël L. Cartron, Paul H. Rieger, Florian Mostegel, Thomas Griesser |
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| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Materials science Biomolecule Biomedical Engineering Nanotechnology General Chemistry General Medicine Sulfonic acid chemistry.chemical_compound Sulfonate chemistry Polymerization Thiol Surface modification General Materials Science Reactivity (chemistry) Protein adsorption |
| Zdroj: | Journal of Materials Chemistry B. 3:4431-4438 |
| ISSN: | 2050-7518 2050-750X |
| Popis: | Thiol-based chemistry provides a mild and versatile tool for surface functionalization. In the present work, mercaptosilane films were patterned by utilizing UV-induced photo-oxidation of the thiol to yield sulfonate groups via contact and interferometric lithography (IL). These photo-generated sulfonic acid groups were used for selective immobilization of amino-functionalized molecules after activation with triphenylphosphine ditriflate (TPPDF). Moreover, protein-resistant poly(oligoethyleneglycolmethacrylate) (POEGMA) brushes were grown from the intact thiol groups by a surface-induced polymerization reaction. Exploiting both reactions it is possible to couple amino-labelled nitrilotriacetic acid (NH2-NTA) to sulfonate-functionalized regions, enabling the site-specific binding of green fluorescent protein (GFP) to regions defined lithographically, while exploiting the protein-resistant character of POEGMA brushes to prevent non-specific protein adsorption to previously masked areas. The outstanding reactivity of thiol groups paves the way towards novel strategies for the fabrication of complex protein nanopatterns beyond thiol–ene chemistry. |
| Databáze: | OpenAIRE |
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