Decrease in CD9 content and reorganization of microvilli may contribute to the oolemma block to sperm penetration during fertilization of mouse oocyte
| Autor: | Marek Maleszewski, Eliza Żyłkiewicz, Julita Nowakowska |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
endocrine system Tetraspanin 29 Mice Human fertilization Tetraspanin Antigens CD medicine Animals Sperm-Ovum Interactions Membrane Glycoproteins Zygote Microvilli Chemistry Cell Membrane Oocyte activation Cell Biology Parthenogenesis Anatomy Oocyte Cell biology medicine.anatomical_structure embryonic structures Oocytes Ultrastructure Immunostaining Developmental Biology |
| Zdroj: | Zygote. 18:195-201 |
| ISSN: | 1469-8730 0967-1994 |
| DOI: | 10.1017/s0967199409990189 |
| Popis: | SummaryTetraspanin CD9 is the only protein of the oocyte membrane (oolemma) known to be required for the fusion of gametes during fertilization in the mouse. Using electron microscopy and immunostaining we examined the differences in localization of CD9 between ovulated oocytes, zygotes and parthenogenetically activated eggs (parthenogenotes). Changes in ultrastructure of oolemma, which take place in oocytes after fertilization or artificial activation, were also assessed. We demonstrated that after fertilization the level of CD9 present on microvilli of zygote was two times lower than its level on the oolemma of the oocyte. In addition, we showed that the distribution of microvilli is less uniform in the zygotes than in the unfertilized oocytes. We propose that the changes of microvilli distribution and their CD9 content are responsible for the development of the oocyte membrane block to sperm penetration. |
| Databáze: | OpenAIRE |
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