Molecular targets of antimicrobial photodynamic therapy identified by a proteomic approach
| Autor: | Enrico Teardo, Elena Reddi, Giorgio Arrigoni, Lucia Puricelli, Anna Segalla, Ryan Dosselli, Cinzia Franchin, Paolo Tessari, Renato Millioni |
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| Rok vydání: | 2012 |
| Předmět: |
Proteomics
Staphylococcus aureus Porphyrins Antibiotic resistance medicine.medical_treatment Biophysics Photodynamic therapy Biology medicine.disease_cause Biochemistry Bacterial Proteins Drug Resistance Bacterial medicine Photosensitizer Gel electrophoresis Photosensitizing Agents Methicillin-resistant Staphylococcus aureus Cationic porphyrin Staphylococcal Infections Antimicrobial Molecular biology Oxidative Stress Photochemotherapy Membrane protein Oxidative stress |
| Zdroj: | Journal of Proteomics. 77:329-343 |
| ISSN: | 1874-3919 |
| Popis: | Antimicrobial photodynamic therapy (PDT) is a promising tool to combat antibiotic-resistant bacterial infections. During PDT, bacteria are killed by reactive oxygen species generated by a visible light absorbing photosensitizer (PS). We used a classical proteomic approach that included two-dimensional gel electrophoresis and mass spectrometry analysis, to identify some proteins of Staphylococcus aureus that are damaged during PDT with the cationic PS meso-tetra-4-N-methyl pyridyl porphine (T4). Suspensions of S. aureus cells were incubated with selected T4 concentrations and irradiated with doses of blue light that reduced the survival to about 60% or 1%. Proteomics analyses of a membrane proteins enriched fraction revealed that these sub-lethal PDT treatments affected the expression of several functional classes of proteins, and that this damage is selective. Most of these proteins were found to be involved in metabolic activities, in oxidative stress response, in cell division and in the uptake of sugar. Subsequent analyses revealed that PDT treatments delayed the growth and considerably reduced the glucose consumption capacity of S. aureus cells. This investigation provides new insights towards the characterization of PDT induced damage and mechanism of bacterial killing using, for the first time, a proteomic approach. |
| Databáze: | OpenAIRE |
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