Blood donor‐derived buffy coat to produce platelets in vitro
Autor: | Matthew T. Rondina, Irene Marini, Tamam Bakchoul, Stefanie Nowak-Harnau, Flavianna Rigoni, Karina Althaus, Jan Zlamal, Lisann Pelzl |
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Rok vydání: | 2019 |
Předmět: |
Blood Platelets
CD34 Blood Donors Buffy coat 030204 cardiovascular system & hematology Flow cytometry 03 medical and health sciences 0302 clinical medicine medicine Humans Platelet Progenitor cell medicine.diagnostic_test Chemistry Hematology General Medicine Flow Cytometry Hematopoietic Stem Cells Molecular biology In vitro Hematopoiesis Haematopoiesis Platelet transfusion Blood Buffy Coat Megakaryocytes 030215 immunology |
Zdroj: | Vox Sanguinis. 115:94-102 |
ISSN: | 1423-0410 0042-9007 |
DOI: | 10.1111/vox.12863 |
Popis: | Background and objectives Platelet transfusion is a standard medical therapy used to treat several bleeding disorders. However, a critical drawback is the dependency on donor-derived platelets, which leads to concerns like insufficient availability and immunological complications. In vitro platelet production from hematopoietic progenitor cells (CD34) may represent a reasonable solution. Materials and methods CD34+ cells were isolated from either buffy coat or peripheral blood and compared in terms of platelet production in vitro. The number and the quality of magnetically isolated CD34+ cells and their capability to differentiate into mature megakaryocytes were investigated using flow cytometry. Additionally, the functionality of megakaryocytes in term of in vitro platelet production was tested. Results Similar purity and quantity of CD34+ cells was found after their isolation from both cell sources. In contrast, after 6 days of culture, enhanced number of CD34+ cells isolated from buffy coat compared with peripheral blood was observed (5·3 x 106 vs. 3·0 x 106, respectively). Interestingly, despite a comparable nuclear maturation phenotype, the yield of platelets released from buffy coat-derived megakaryocytes was significantly higher than from peripheral blood cells (platelet yield pro MK: 7·2 vs. 2·7, respectively). Importantly, platelets produced from buffy coat-derived cells could be activated by agonists. Conclusion Haematopoietic progenitor cells isolated from buffy coat have increased yield of platelets released from mature megakaryocytes and enhanced in vitro functionality, compared with peripheral blood-derived cells. Our study, suggests that buffy coat, obtained during blood donation processing, might be a promising source of megakaryocytes for in vitro platelet production. |
Databáze: | OpenAIRE |
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