Investigating genetic diversity in sapucaia using inter simple sequence repeat markers
| Autor: | Sérgio Emílio dos Santos Valente, R.C. Borges, Paulo Sarmanho da Costa Lima, F.M.G. Santos, Maria Clideana Cabral Maia |
|---|---|
| Přispěvatelé: | R. C. BORGES, UFPI, F. M. G. SANTOS, UNINOVAFAPI, MARIA CLIDEANA CABRAL MAIA, CPAMN, PAULO SARMANHO DA COSTA LIMA, CPAMN, S. E. S. VALENTE, UFPI. |
| Rok vydání: | 2016 |
| Předmět: |
Genetic Markers
0106 biological sciences Germplasm Veterinary medicine Variabilidade genética Sapucaia Biology Genes Plant 010603 evolutionary biology 01 natural sciences Trees Gene flow Genetic variation Botany Genetics Plant breeding Genetic variability Molecular Biology Phylogeny Lecythis Pisonis Genetic diversity Polymorphism Genetic Models Genetic Bayes Theorem Sequence Analysis DNA General Medicine Banco de Germoplasma 0104 chemical sciences 010404 medicinal & biomolecular chemistry Genetic marker Microsatellite Microsatellite Repeats |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| ISSN: | 1676-5680 |
| Popis: | Sapucaia is a tree species originating from the Brazilian Amazon and is widely distributed in Brazil, especially in the mid-north region (Piaui and Maranhao states). Its seeds are rich in calories and proteins, and possess great potential for commercialization. Little is known about the genetic variability in the germplasm of most Lecythis species. Here, 11 inter-simple sequence repeat primers were used to estimate the genetic variability among 17 accessions, and to determine the levels of genetic variation and the standards of population structure in sapucaia. The accessions were obtained from the active germplasm bank (AGB) of Embrapa Meio-Norte, Teresina, PI, Brazil, and corresponded to four occurrence areas. Ninety-six loci were analyzed among the studied individuals. High variation was found at the species level, where the percentage of polymorphic bands was 94.79%, Nei's genetic diversity (h) was 0.3110, and Shannon's index (I) was 0.4732. In the analyzed populations, the percentage polymorphism ranged from 20.83 to 94.79%, Nei's genetic diversity ranged from 0.0863 to 0.2969, and Shannon's index ranged from 0.1260 to 0.4457. Significant genetic differentiation was detected among the populations (ΦST = 10.66%); however, the greatest genetic differentiation was found within the populations (89.34%), between which there was an intermediate level of gene flow (Nm = 1.10). Accessions BGS 2 and BGS 4 were the most divergent, whereas accessions BGS 14 and BGS 15 were the most similar. Therefore, sapucaia analyzed from the AGB present an elevated level of genetic diversity and may have potential use in genetic breeding programs. |
| Databáze: | OpenAIRE |
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