Reduced ob mRNA in Hypophysectomised Rats Is Not Restored by Growth Hormone (GH), but further Suppressed by Exogenously Administered Insulin-like Growth Factor (IGF) I
Autor: | W. Moritz, Jürgen Zapf, M.A. Gosteli-Peter, Ernst Rudolf Prof Dr Froesch, M. Böni-Schnetzler |
---|---|
Rok vydání: | 1996 |
Předmět: |
Leptin
Male medicine.medical_specialty Hypophysectomy Transcription Genetic medicine.medical_treatment Mrna expression Molecular Sequence Data Serum insulin Biophysics Growth hormone Polymerase Chain Reaction Biochemistry law.invention Insulin-like growth factor Reference Values law Internal medicine medicine Animals Humans Insulin RNA Messenger Insulin-Like Growth Factor I Molecular Biology DNA Primers Messenger RNA Base Sequence Chemistry Body Weight Proteins Rats Inbred Strains Cell Biology Recombinant Proteins Rats Endocrinology Adipose Tissue Mrna level Growth Hormone Protein Biosynthesis Recombinant DNA DNA Probes |
Zdroj: | Biochemical and Biophysical Research Communications. 225:296-301 |
ISSN: | 0006-291X |
DOI: | 10.1006/bbrc.1996.1169 |
Popis: | To examine whether GH and IGF-I participate in the regulation of obese (ob) mRNA expression we determined ob mRNA levels in epididydimal fat pads of hypophysectomised (hypox) rats, hypox rats treated with recombinant human (rh) GH or rhIGF-I and normal, weight-matched controls. We found that 1. ob mRNA was markedly suppressed after hypophysectomy (37 ± 25% of controls), 2. GH infusion had no effect on ob mRNA, but stimulated IGF-I mRNA in fat pads, 3. IGF-I treatment further suppressed ob mRNA (3.5% ± 0.6% of controls) and 4. serum insulin levels were decreased in all hypox groups (11.2 to 15.9% of controls). In conclusion, exogenous and GH-induced IGF-I differ in their effects on ob mRNA expression and GH is unable to restore ob mRNA towards normal at low insulin levels. |
Databáze: | OpenAIRE |
Externí odkaz: |