Copy Number Variation of the GeneNCF1Is Associated with Rheumatoid Arthritis
| Autor: | Patrik Medstrand, Åsa Johansson, Annika Nerstedt, Rikard Holmdahl, Anna-Karin B. Lindqvist, Peter Olofsson, Lina Olsson |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Adolescent DNA Copy Number Variations Genotype Physiology Clinical Biochemistry Gene Dosage Biology Bioinformatics Polymorphism Single Nucleotide Biochemistry Gene dosage Arthritis Rheumatoid Young Adult Gene Order medicine Humans Copy-number variation Allele Molecular Biology Gene Alleles Aged General Environmental Science Case-control study NADPH Oxidases Cell Biology Neutrophil cytosolic factor 1 Middle Aged medicine.disease Case-Control Studies Rheumatoid arthritis General Earth and Planetary Sciences Female Reactive Oxygen Species |
| Zdroj: | Antioxidants & Redox Signaling. 16:71-78 |
| ISSN: | 1557-7716 1523-0864 |
| DOI: | 10.1089/ars.2011.4013 |
| Popis: | The aim of this study was to investigate genetic variants in the gene neutrophil cytosolic factor 1 (NCF1) for association with rheumatoid arthritis (RA). In rodent models, a single-nucleotide polymorphism (SNP) in Ncf1 has been shown to be a major locus regulating severity of arthritis. Ncf1 encodes one of five subunits of the NADPH oxidase complex. In humans the genomic structure of NCF1 is complex, excluding it from genome-wide association screens and complicating genetic analysis. In addition to copy number variation of NCF1, there are also two nonfunctional pseudogenes, nearly identical in sequence to NCF1. We have characterized copy number variation and SNPs in NCF1, and investigated these variants for association with RA.We find that RA patients are less likely to have an increased copy number of NCF1, 7.6%, compared with 11.6% in controls; p=0.037. We also show that the T-allele of NCF1-339 (rs13447) is expressed in NCF1 and significantly reduces reactive oxygen species production.This is the first finding of genetic association of NCF1 with RA. The detailed characterization of genetic variants in NCF1 also helps elucidate the complexity of the NCF1 gene.These data suggest that an increased copy number of NCF1 can be protective against developing RA and add support to previous findings of a role of NCF1 and the phagocyte NADPH oxidase complex in RA pathogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|