A New Cell Block Method for Multiple Immunohistochemical Analysis of Circulating Tumor Cells in Patients with Liver Cancer

Autor: Joong-Won Park, Bohyun Kim, Hee Jin Chang, Soo Jeong Nam, Eun Kyung Hong, Hyun Yang Yeo
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
Cancer Research
Pathology
Hepatocellular carcinoma
Cell Separation
Blood cell
chemistry.chemical_compound
0302 clinical medicine
Circulating tumor cell
Aged
80 and over
biology
Liver Neoplasms
Epithelial cell adhesion molecule
Middle Aged
Epithelial Cell Adhesion Molecule
Neoplastic Cells
Circulating
Immunohistochemistry
Gene Expression Regulation
Neoplastic
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Original Article
Female
alpha-Fetoproteins
Antibody
Liver cancer
Adult
medicine.medical_specialty
Carcinoma
Hepatocellular
Glypican 3
03 medical and health sciences
Cytokeratin
Glypicans
Antigens
Neoplasm
Biomarkers
Tumor
medicine
Humans
Cell Lineage
Aged
Keratin-18
business.industry
Mucin-1
medicine.disease
digestive system diseases
Circulating neoplastic cells
030104 developmental biology
chemistry
biology.protein
business
Zdroj: Cancer Research and Treatment : Official Journal of Korean Cancer Association
ISSN: 2005-9256
1598-2998
Popis: Purpose We developed a new method of detecting circulating tumor cells (CTCs) in liver cancer patients by constructing cell blocks from peripheral blood cells, including CTCs, followed by multiple immunohistochemical analysis. Materials and methods Cell blockswere constructed from the nucleated cell pellets of peripheral blood afterremoval of red blood cells. The blood cell blocks were obtained from 29 patients with liver cancer, and from healthy donor blood spikedwith seven cell lines. The cell blocks and corresponding tumor tissues were immunostained with antibodies to seven markers: cytokeratin (CK), epithelial cell adhesion molecule (EpCAM), epithelial membrane antigen (EMA), CK18, α-fetoprotein (AFP), Glypican 3, and HepPar1. Results The average recovery rate of spiked SW620 cells from blood cell blocks was 91%. CTCs were detected in 14 out of 29 patients (48.3%); 11/23 hepatocellular carcinomas (HCC), 1/2 cholangiocarcinomas (CC), 1/1 combined HCC-CC, and 1/3 metastatic cancers. CTCs from 14 patients were positive for EpCAM (57.1%), EMA (42.9%), AFP (21.4%), CK18 (14.3%), Gypican3 and CK (7.1%, each), and HepPar1 (0%). Patients with HCC expressed EpCAM, EMA, CK18, and AFP in tissue and/or CTCs, whereas CK, HepPar1, and Glypican3 were expressed only in tissue. Only EMA was significantly associated with the expressions in CTC and tissue. CTC detection was associated with higher T stage and portal vein invasion in HCC patients. Conclusion This cell block method allows cytologic detection and multiple immunohistochemical analysis of CTCs. Our results show that tissue biomarkers of HCC may not be useful for the detection of CTC. EpCAM could be a candidate marker for CTCs in patients with HCC.
Databáze: OpenAIRE
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