DNA adsorption measured with ultra-thin film organic field effect transistors
| Autor: | Eva Bystrenova, M. Spijkman, Paolo Annibale, Massimo Facchini, Santiago David Quiroga, Dago M. de Leeuw, Sepas Setayesh, Fabio Biscarini, Pablo Stoliar |
|---|---|
| Přispěvatelé: | Zernike Institute for Advanced Materials |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Naphthacenes
Transistors Electronic Base pair Biomedical Engineering Biophysics Analytical chemistry Pentacene Biosensing Techniques Microscopy Atomic Force Sensitivity and Specificity chemistry.chemical_compound Adsorption Monolayer Electrochemistry Thin film DNA Organic field effect transistor Pentacene Biosensors HYBRIDIZATION MICROSCOPY DIFFUSION SENSORS DNA Organicfieldeffecttransistor Biosensors Organic field-effect transistor Chemistry business.industry General Medicine Nanostructures Semiconductor Field-effect transistor business Organic field effect transistor Biotechnology |
| Zdroj: | Biosensors & bioelectronics 24 (2009): 2935–2938. doi:10.1016/j.bios.2009.02.003 info:cnr-pdr/source/autori:P. Stoliar (1), E. Bystrenova (1), S. D. Quiroga (1), P. Annibale (1), M. Facchini (1), M. Spijkman (2), S. Setayesh (2), D. de Leeuw (2) and F. Biscarini (1)/titolo:DNA adsorption measured with ultra-thin film organic field effect transistors/doi:10.1016%2Fj.bios.2009.02.003/rivista:Biosensors & bioelectronics/anno:2009/pagina_da:2935/pagina_a:2938/intervallo_pagine:2935–2938/volume:24 Biosensors and Bioelectronics, 24(9), 2935-2938 ESF-EMBO Symposium, Biological surfaces and interfaces, Sant Feliu de Guixols, 2009 info:cnr-pdr/source/autori:E. Bystrenova, P. Stoliar, S. D. Quiroga, P. Annibale, M. Facchini, M. Spijkman, S. Setayesh, D. de Leeuw and F. Biscarini/congresso_nome:ESF-EMBO Symposium, Biological surfaces and interfaces/congresso_luogo:Sant Feliu de Guixols/congresso_data:2009/anno:2009/pagina_da:/pagina_a:/intervallo_pagine |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2009.02.003 |
| Popis: | Organic ultra-thin film field effect transistors (FET) are operated as label-free sensors of deoxyribonucleic acid (DNA) adsorption. Linearized plasmid DNA molecules (4361 base pairs) are deposited from a solution on two monolayers thick pentacene FET. The amount of adsorbed DNA is measured by AFM and correlated to the concentration of the solution. Electrical characteristics on the dried DNA/pentacene FETs were studied as a function of DNA concentration in the solution. Shift of the pinch-off voltage across a wide range of DNA concentration, from very diluted to highly concentrated, is observed. It can be ascribed to additional positive charges in the semiconductor induced by DNA at a rate of one charge for every 200 base pairs. The sensitivity 74 ng/cm 2 , corresponding to 650 ng/ml, is limited by the distribution of FET parameters upon repeated cycles, and is subjected to substantial improvement upon standardization. Our work demonstrates the possibility to develop label-free transducers suitable to operate in regimes of high molecular entanglement. |
| Databáze: | OpenAIRE |
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