Coordinated collective migration and asymmetric cell division in confluent human keratinocytes without wounding

Autor: Kim Alexander Tønseth, Jens Eriksson, Alexander D. Rowe, Liesbeth M. C. Janssen, Catherine Jackson, Anna Połeć, Tor Paaske Utheim, Pernille Blicher, Stig Ove Bøe, Anna Ulrika Lång, Marijke Valk, Emma Lång
Přispěvatelé: Applied Physics and Science Education, Soft Matter and Biological Physics
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Keratinocytes
0301 basic medicine
Cell division
Cellular differentiation
General Physics and Astronomy
Lysosomes/metabolism
Epithelium
Cohort Studies
Blood serum
Cell Movement
Epidermal growth factor
Cell polarity
Asymmetric cell division
lcsh:Science
Microscopy
ErbB Receptors/metabolism
Tumor
Microscopy
Confocal
Multidisciplinary
Chemistry
Cell Cycle
Cell Polarity
Cell Differentiation
Cell cycle
Cell biology
ErbB Receptors
Confocal
Cell Division
Signal Transduction
Science
Mitosis
Resting Phase
Cell Cycle
Article
General Biochemistry
Genetics and Molecular Biology
Cell Line
03 medical and health sciences
Cell Line
Tumor
Humans
Keratinocytes/cytology
Asymmetric Cell Division
G1 Phase
General Chemistry
Epithelium/metabolism
030104 developmental biology
Resting Phase
lcsh:Q
Epidermis
Lysosomes
Epidermis/metabolism
HeLa Cells
Zdroj: Nature Communications, Vol 9, Iss 1, Pp 1-15 (2018)
Nature Communications, 9(1):3665. Nature Publishing Group
Nature Communications
ISSN: 2041-1723
Popis: Epithelial sheet spreading is a fundamental cellular process that must be coordinated with cell division and differentiation to restore tissue integrity. Here we use consecutive serum deprivation and re-stimulation to reconstruct biphasic collective migration and proliferation in cultured sheets of human keratinocytes. In this system, a burst of long-range coordinated locomotion is rapidly generated throughout the cell sheet in the absence of wound edges. Migrating cohorts reach correlation lengths of several millimeters and display dependencies on epidermal growth factor receptor-mediated signaling, self-propelled polarized migration, and a G1/G0 cell cycle environment. The migration phase is temporally and spatially aligned with polarized cell divisions characterized by pre-mitotic nuclear migration to the cell front and asymmetric partitioning of nuclear promyelocytic leukemia bodies and lysosomes to opposite daughter cells. This study investigates underlying mechanisms contributing to the stark contrast between cells in a static quiescent state compared to the long-range coordinated collective migration seen in contact with blood serum.
Epithelial sheet migration requires polarized and coordinated cell movement. Here, the authors demonstrate serum-activated collective migration followed by polarized asymmetric cell divisions in otherwise quiescent human keratinocyte monolayers in the absence of wound edges.
Databáze: OpenAIRE
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