Separation of digesta fractions complicates estimation of ileal digestibility using marker methods with Cr2 O3 and cobalt-ethylenediamine tetraacetic acid in broiler chickens
| Autor: | Walter J. J. Gerrits, S. de Vries, Mirjam A. Kabel, A.M. Pustjens, R. P. Kwakkel, Wouter H. Hendriks |
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| Rok vydání: | 2014 |
| Předmět: |
Dietary Fiber
Rapeseed chemistry.chemical_element Ileum Fractionation Biology Polysaccharide Chromium Animal science Chromium Compounds medicine Animals Fiber Edetic Acid chemistry.chemical_classification Meal Chromatography Animal Nutrition Sciences Broiler General Medicine Animal Feed Diet Gastrointestinal Tract medicine.anatomical_structure chemistry Animal Nutritional Physiological Phenomena Digestion Female Animal Science and Zoology Chickens Biomarkers |
| Zdroj: | Poultry Science. 93:2010-2017 |
| ISSN: | 0032-5791 |
| DOI: | 10.3382/ps.2013-03845 |
| Popis: | Marker methodologies to measure ileal and total tract digestibilities of diets varying in content and degradability of dietary fiber in broiler chickens were evaluated. Chromium sesquioxide (Cr2O3) and cobalt-ethylenediamine tetraacetic acid (Co-EDTA) were used as markers of solid and soluble fractions, respectively, and compared with digestibility values obtained with the total collection method. Groups of broilers (n = 17, 11 broilers/group) were assigned to a low-fiber diet or 1 of 2 high-fiber diets, the latter 2 containing 35% rapeseed meal (RSM). Pectolytic enzymes were added to one RSM diet to improve degradability of the fiber fraction. Excreta were quantitatively collected for 96 h, and contents from ileum and ceca were collected at slaughter at 29, 30, or 31 d of age. Chromium recovery in excreta ranged between 86 and 95%, whereas cobalt recovery was considerably lower (66 to 70%). Chromium:cobalt ratio was higher in ileal digesta than in feed. Hardly any chromium was found in the ceca, indicating that separation of the marker and specific digesta fractions occurs. Estimates of apparent total tract digestibility (ATTD) were lower when calculated using the marker method compared with the total collection method, particularly in high-fiber diets. Using Cr2O3 as a marker, differences were relatively small and effects due to enzyme addition were generally similar. Using Co-EDTA as a marker, ATTD of all components were lower compared with values obtained using the collection method (3 to 45 percentage units, P |
| Databáze: | OpenAIRE |
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