Induction of protective immunity to bovine herpesvirus type 1 in cattle by intranasal administration of replication-defective human adenovirus type 5 expressing glycoprotein gC or gD
| Autor: | Frédéric Schynts, Jean D’Offay, Nathalie Vanderheijden, Isabelle Deprez, Etienne Thiry, Sacha Gogev, Micheline Adam, Marc Eloit, Mylène Lemaire |
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| Rok vydání: | 2002 |
| Předmět: |
Herpesvirus Vaccines
Antibodies Viral Vaccines Attenuated Virus Replication medicine.disease_cause Virus Cell Line Interferon-gamma Viral Proteins medicine Animals Humans Cloning Molecular Neutralizing antibody Administration Intranasal Herpesvirus 1 Bovine Vaccines Synthetic Attenuated vaccine General Veterinary General Immunology and Microbiology biology Adenoviruses Human Public Health Environmental and Occupational Health biology.organism_classification Virology Bovine herpesvirus 1 Adenoviridae Mastadenovirus Nasal Mucosa Infectious Diseases biology.protein Molecular Medicine Cattle Immunization Nasal administration Rabbits Antibody |
| Zdroj: | Vaccine. 20:1451-1465 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(01)00458-3 |
| Popis: | Replication-defective human adenoviruses type 5 (HAd5) expressing the bovine herpesvirus type 1 (BHV-1) glycoprotein gC or gD under the control of the human cytomegalovirus immediate-early promoter/enhancer (AdCMVgC or AdCMVgD) or the 5′ regulatory region of the human desmin gene (AdDESMgC or AdDESMgD) were generated. A preliminary experiment performed on rabbits showed that the intranasal administration of AdCMV elicited higher levels of BHV-1 neutralizing antibodies than the intramuscular administration of AdDESM. The obtained results allowed to select the replication-defective AdCMVgC and AdCMVgD for further assessment of their potential as a recombinant vaccine in cattle. Calves were injected intranasally twice 3 weeks apart with either AdCMVgC or AdCMVgD or a combination of these two recombinants or a commercially available live vaccine for comparison. The highest BHV-1 neutralizing antibody titres were obtained with AdCMVgD followed by the live vaccine and to a lower extent with the combination of the two recombinants (AdCMVgC+AdCMVgD). Calves were protected against intranasal BHV-1 challenge performed 3 weeks after the second immunization. In view of the obtained results, recombinant HAd5 may be developed as an intranasal vaccine vector in cattle administrated either alone or sequentially with non-human adenovirus-based vectors. |
| Databáze: | OpenAIRE |
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