Stimulation of skeletal-derived cell cultures by different electric field intensities is cell-specific
| Autor: | Henryk Fischler, Dalia Somjen, J. Levy, Rafi Korenstein, Itzhak Binderman, Zvi Shimshoni |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Cell type
medicine.medical_specialty Population Field strength Calvaria Biology Bone and Bones Internal medicine Bone cell Cyclic AMP medicine Animals Growth Plate education Molecular Biology Cells Cultured education.field_of_study Osteoblasts Cell growth Osteoblast DNA Cell Biology Fibroblasts Electric Stimulation Rats Cartilage Endocrinology medicine.anatomical_structure Parathyroid Hormone Cell culture Biophysics Calcium |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 844:273-279 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/0167-4889(85)90127-2 |
| Popis: | Pulsed electric stimulation, coupled capacitively to different cell cultures of skeletal origin, caused immediate changes in the cellular levels of cyclic AMP and a later enhanced DNA synthesis. Changes both in cyclic AMP level and DNA synthesis were correlated with the strength of the applied electric field. Cultures of calvaria bone cells which contain mainly two cell types, parathyroid hormone responsive cells (osteoblast-like) and prostaglandin E2 responsive cells (fibroblast-like), respond to both low (13 V/cm) and to high (54 V/cm) electric field strength, with no response at intermediate (24 V/cm) field strength. Rat epiphyseal cartilage responded like bone cells both to low and high field intensities, while rat condylar cartilage responded only to the intermediate field strength. Moreover, subcultures of calvaria bone cells, which lost their osteoblastic phenotype expression during subculturing, were responsive only to low field strength. On the other hand, osteoblast-enriched cultures, derived from calvaria bone grown in low calcium, were responsive only to the high field strength. These findings suggest that the response to various electric field intensities is cell-specific and might be used as an additional parameter to characterize cell types. Our study points to the possibility that when exposing a whole organ to an electrical stimulation it is possible to affect specifically only one cell population out of the many cell types existing in the organ. |
| Databáze: | OpenAIRE |
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