Evolutionary conserved role of eukaryotic translation factor eIF5A in the regulation of actin-nucleating formins
| Autor: | Esther Gamero, Ana Domingo-Muelas, Isabel Fariñas, Tianlu Li, Verónica Muñoz-Soriano, Paula Alepuz, Alexandra Bizy, Nuria Paricio |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Fluorescent Antibody Technique lcsh:Medicine macromolecular substances Biology Article Mice Eukaryotic cells 03 medical and health sciences Eukaryotic translation Cell Movement Peptide Initiation Factors Citosquelet Protein biosynthesis Animals Protein Interaction Domains and Motifs lcsh:Science Cytoskeleton Actin Multidisciplinary Cèl·lules eucariotes Microfilament Proteins fungi lcsh:R Gene Expression Regulation Developmental RNA-Binding Proteins Translation (biology) Biological Evolution Actins Dorsal closure Cell biology Elongation factor 030104 developmental biology Protein Biosynthesis Formins Mutation biology.protein Drosophila lcsh:Q EIF5A |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) Scientific Reports Recercat. Dipósit de la Recerca de Catalunya instname Dipòsit Digital de la UB Universidad de Barcelona |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-10057-y |
| Popis: | Elongation factor eIF5A is required for the translation of consecutive prolines, and was shown in yeast to translate polyproline-containing Bni1, an actin-nucleating formin required for polarized growth during mating. Here we show that Drosophila eIF5A can functionally replace yeast eIF5A and is required for actin-rich cable assembly during embryonic dorsal closure (DC). Furthermore, Diaphanous, the formin involved in actin dynamics during DC, is regulated by and mediates eIF5A effects. Finally, eIF5A controls cell migration and regulates Diaphanous levels also in mammalian cells. Our results uncover an evolutionary conserved role of eIF5A regulating cytoskeleton-dependent processes through translation of formins in eukaryotes. |
| Databáze: | OpenAIRE |
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