Lipids status and copper in a single astrocyte of the rat model for amyotrophic lateral sclerosis: Correlative synchrotron‐based X‐ray and infrared imaging
| Autor: | Tanja Dučić, Si Chen, Stefan Stamenković, Martin Kreuzer, Pavle R. Andjus |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
SOD1
Excitotoxicity General Physics and Astronomy Mice Transgenic Protein aggregation medicine.disease_cause 01 natural sciences General Biochemistry Genetics and Molecular Biology 010309 optics Mice Superoxide Dismutase-1 0103 physical sciences medicine Animals General Materials Science Amyotrophic lateral sclerosis Chemistry X-Rays Amyotrophic Lateral Sclerosis 010401 analytical chemistry General Engineering Neurodegenerative Diseases General Chemistry Compartmentalization (fire protection) medicine.disease Lipids Rats 0104 chemical sciences Disease Models Animal medicine.anatomical_structure Astrocytes Axoplasmic transport Biophysics Copper Synchrotrons Oxidative stress Astrocyte |
| Zdroj: | Journal of Biophotonics. 13 |
| ISSN: | 1864-0648 1864-063X |
| DOI: | 10.1002/jbio.202000069 |
| Popis: | Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease, causing death of motor neurons controlling voluntary muscles. The pathological mechanisms of the disease are only partially understood. The hSOD1-G93A ALS rat model is characterized by an overexpression of human mutated SOD1, causing increased vulnerability by forming intracellular protein aggregates, inducing excitotoxicity, affecting oxidative balance and disturbing axonal transport. In this study we followed the bio-macromolecular organic composition and compartmentalization together with trace metal distribution in situ in single astrocytes from the ALS rat model and compared them to the control astrocytes from nontransgenic littermates by simultaneous use of two synchrotron radiation-based methods: Fourier transform infrared microspectroscopy (SR-FTIR) and hard X-ray fluorescence microscopy (XRF). We show that ALS cells contained more Cu, which colocalized with total lipids, increased carbonyl groups and oxidized lipids, thus implying direct involvement of Cu in oxidative stress of lipidic components without direct connection to protein aggregation in situ. |
| Databáze: | OpenAIRE |
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