Monitoring seasonal influenza A evolution: rapid 2009 pandemic H1N1 surveillance with an reverse transcription-polymerase chain reaction/electro-spray ionization mass spectrometry assay
| Autor: | Yu Hsiang Hsieh, Larisa V. Gubareva, Sudhir Penugonda, Richard E. Rothman, Christian Massire, Teresa R. Zembower, Varough M. Deyde, Charlotte A. Gaydos, David Metzgar, Kevin Jeng, Lawrence B. Blyn, Rangarajan Sampath, Justin Hardick |
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| Rok vydání: | 2012 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Time Factors Biology medicine.disease_cause Sensitivity and Specificity Virus Article Evolution Molecular Influenza A Virus H1N1 Subtype Virology Pandemic Genotype Influenza Human Influenza A virus medicine Humans Typing Genotyping Pandemics Genetic diversity Reverse Transcriptase Polymerase Chain Reaction Strain (biology) virus diseases Infectious Diseases Population Surveillance Seasons |
| Zdroj: | Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 54(4) |
| ISSN: | 1873-5967 |
| Popis: | Background The emergence of the pandemic H1N1 influenza strain in 2009 reinforced the need for improved influenza surveillance efforts. A previously described influenza typing assay that utilizes RT-PCR coupled to electro-spray ionization mass spectrometry (ESI-MS) played an early role in the discovery of the pandemic H1N1 influenza strain, and has potential application for monitoring viral genetic diversity in ongoing influenza surveillance efforts. Objectives To determine the analytical sensitivity of RT-PCR/ESI-MS influenza typing assay for identifying the pandemic H1N1 strain and describe its ability to assess viral genetic diversity. Study design Two sets of pandemic H1N1 samples, 190 collected between April and June of 2009, and 69 collected between October 2009 and January 2010, were processed by the RT-PCR/ESI-MS influenza typing assay, and the spectral results were compared to reference laboratory results and historical sequencing data from the Nucleotide Database of the National Center for Biotechnology Information (NCBI). Results Strain typing concordance with reference standard testing was 100% in both sample sets, and the assay demonstrated a significant increase in influenza genetic diversity, from 10.5% non-wildtype genotypes in early samples to 69.9% in late samples (P |
| Databáze: | OpenAIRE |
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