A ROS Repressor-mediated Binary Regulation System for Control of Gene Expression in Transgenic Plants
| Autor: | Ulrike A. Schäfer, Dwayne D. Hegedus, Nicholas J. Bate, Abdelali Hannoufa |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Operator Regions
Genetic Operator (biology) TATA box Transgene Molecular Sequence Data Arabidopsis Repressor Biology Bacterial Proteins Gene Expression Regulation Plant Genes Reporter Tobacco Gene expression Genetics Promoter Regions Genetic Gene Glucuronidase Cell Nucleus Regulation of gene expression Reporter gene Base Sequence fungi food and beverages Plants Genetically Modified Molecular biology DNA-Binding Proteins Repressor Proteins Agrobacterium tumefaciens Animal Science and Zoology Sequence Alignment Agronomy and Crop Science Biotechnology |
| Zdroj: | Transgenic Research. 13:109-118 |
| ISSN: | 0962-8819 |
| Popis: | We describe a novel binary system to control transgene expression in plants. The system is based on the prokaryotic repressor, ROS, from Agrobacterium tumefaciens, optimized for plant codon usage and for nuclear targeting (synROS). The ROS protein bound in vitro to double stranded DNA comprising the ROS operator sequence, as well as to single stranded ROS operator DNA sequences, in an orientation-independent manner. A synROS-GUS fusion protein was localized to the nucleus, whereas wtROS-GUS fusion remained in the cytoplasm. The ability of synROS to repress transgene expression was validated in transgenic Arabidopsis thaliana and Brassica napus. When expressed constitutively under the actin2 promoter, synROS repressed the expression of the reporter gene gusA linked to a modified CaMV35S promoter containing ROS operator sequences in the vicinity of the TATA box and downstream of the transcription initiation signal. Repression ranged from 32 to 87% in A. thaliana, and from 23 to 76% in B. napus. These results are discussed in relation to the potential application of synROS in controlling the expression of transgenes and endogenous genes in plants and other organisms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink