Identification of an aldehyde oxidase involved in indole-3-acetic acid synthesis in Bombyx mori silk gland
| Autor: | Mami Takei, Yoshiaki Kouzuma, Syota Kogure, Chiaki Yokoyama, Yoshihito Suzuki |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Aldehyde Catalysis Substrate Specificity Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Plant Growth Regulators Tandem Mass Spectrometry Auxin Bombyx mori Animals Amino Acid Sequence Molecular Biology Aldehyde oxidase Benzoic acid chemistry.chemical_classification Chromatography Indoleacetic Acids Sequence Homology Amino Acid biology Organic Chemistry Tryptophan food and beverages General Medicine Bombyx biology.organism_classification Aldehyde Oxidase 010602 entomology 030104 developmental biology Enzyme chemistry Electrophoresis Polyacrylamide Gel Spectrophotometry Ultraviolet Indole-3-acetic acid Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 83:129-136 |
| ISSN: | 1347-6947 0916-8451 |
| Popis: | Auxin is thought to be an important factor in the induction of galls by galling insects. We have previously shown that both galling and nongalling insects synthesize indole-3-acetic acid (IAA) from tryptophan (Trp) via two intermediates, indole-3-acetaldoxime (IAOx) and indole-3-acetaldehyde (IAAld). In this study, we isolated an enzyme that catalyzes the last step “IAAld → IAA” from a silk-gland extract of Bombyx mori. The enzyme, designated “BmIAO1”, contains two 2Fe–2S iron–sulfur-cluster-binding domains, an FAD-binding domain, and a molybdopterin-binding domain, which are conserved in aldehyde oxidases. BmIAO1 causes the nonenzymatic conversion of Trp to IAAld and the enzymatic conversion of IAOx to IAA, suggesting that BmIAO1 alone is responsible for IAA production in B. mori. However, a detailed comparison of pure BmIAO1 and the crude silk-gland extract suggested the presence of other enzymes involved in IAA production from Trp. Abbreviations: BA: benzoic acid; CE: collision energy; CXP: collision cell exit potential; DP: declustering potential; IAA: indole-3-acetic acid; IBI1: IAA biosynthetic inhibitor-1; IAAld: indole-3-acetaldehyde; ICA: indole-3-carboxylic acid; IAOx: indole-3-acetaldoxime; IEtOH: indole-3-ethanol; LC–MS/MS: liquid chromatography–tandem mass spectrometry; Trp: tryptophan |
| Databáze: | OpenAIRE |
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