Exploring embryonic germ line development in the water flea, Daphnia magna, by zinc-finger-containing VASA as a marker
| Autor: | Kazunori Sagawa, Hideo Yamagata, Yasuhiro Shiga |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Embryo Nonmammalian Time Factors Amino Acid Motifs Molecular Sequence Data Embryonic Development Germline Moina Crustacea Genetics Animals Amino Acid Sequence RNA Messenger Cloning Molecular Intestinal Mucosa Molecular Biology reproductive and urinary physiology In Situ Hybridization Zinc finger biology Models Genetic Sequence Homology Amino Acid urogenital system Reverse Transcriptase Polymerase Chain Reaction fungi Embryogenesis RNA Embryo Zinc Fingers biology.organism_classification RNA Helicase A Protein Structure Tertiary Germ Cells Daphnia cardiovascular system Female Germ line development RNA Helicases Developmental Biology |
| Zdroj: | Gene expression patterns : GEP. 5(5) |
| ISSN: | 1567-133X |
| Popis: | VASA is an ATP-dependent RNA helicase belonging to the DEAD-box family that, in many organisms, is specifically expressed in germ line cells throughout the life cycle, making it a powerful molecular marker to study germ line development. To obtain further information on germ line development in crustaceans, we cloned VASA cDNAs from three branchiopod species: water fleas Daphnia magna and Moina macrocopa, and brine shrimp Artemia franciscana. RNA helicase domains in branchiopod VASA were highly conserved among arthropod classes. However, N-terminal RNA-binding domains in branchiopod VASA were highly diverged and, unlike other arthropod VASA reported so far, possessed repeats of retroviral-type zinc finger (CCHC) motifs. Raising specific antibodies against Daphnia VASA revealed that the primordial germ cells (PGCs) in this organism segregate at a very early cleavage stage of embryogenesis in parthenogenetic and sexual eggs. Clusters of PGCs then start to migrate inside the embryo and finally settle at both sides of the intestine, the site of future gonad development. RNA analyses suggested that maternally supplied vasa mRNA was responsible for early VASA expression, while zygotic expression started during blastodermal stage of development. |
| Databáze: | OpenAIRE |
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