Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair
| Autor: | Dan E. Meyer, Lori A. Setton, Ashutosh Chilkoti, Helawe Betre |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Polymers and Plastics
Swine Molecular Sequence Data Cell Culture Techniques Bioengineering Pentapeptide repeat Biomaterials Glycosaminoglycan Extracellular matrix Chondrocytes Materials Chemistry Cartilaginous Tissue Animals Coacervate biology Base Sequence Tissue Engineering Chemistry Temperature Chondrogenesis Elastin Cartilage Biochemistry Self-healing hydrogels biology.protein Biophysics Genetic Engineering Peptides Rheology |
| Zdroj: | Biomacromolecules. 3(5) |
| ISSN: | 1525-7797 |
| Popis: | Elastin-like polypeptides (ELPs) are artificial polypeptides with unique properties that make them attractive as a biomaterial for tissue-engineered cartilage repair. ELPs are composed of a pentapeptide repeat, Val-Pro-Gly-Xaa-Gly (Xaa is any amino acid except Pro), that undergo an inverse temperature phase transition. They are soluble in aqueous solution below their transition temperature (T(t)) but aggregate when the solution temperature is raised above their T(t). This study investigates the rheological behavior of an un-cross-linked ELP, below and above its T(t), and also examines the ability of ELP to promote chondrogenesis in vitro. A thermally responsive ELP with a T(t) of 35 degrees C was synthesized using recombinant DNA techniques. The complex shear modulus of the ELP increased by 3 orders of magnitude as it underwent its inverse temperature phase transition, forming a coacervate, or gel-like, ELP phase. Values for the complex shear moduli of the un-cross-linked ELP coacervate are comparable to those reported previously for collagen, hyaluronan, and cross-linked synthetic hydrogels. Cell culture studies show that chondrocytes cultured in ELP coacervate maintain a rounded morphology and their chondrocytic phenotype, characterized by the synthesis of a significant amount of extracellular matrix composed of sulfated glycosaminoglycans and collagen. These results suggest that ELPs demonstrate great potential for use as in situ forming scaffolds for cartilaginous tissue repair. |
| Databáze: | OpenAIRE |
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