Asp3Gly polymorphism affects fatty acid-binding protein 3 intracellular stability and subcellular localization
| Autor: | Yasuhiko Hashida, Hitoshi Yamashita, Hiroshi Shimokata, Tatsuya Kusudo, Fujiko Ando |
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| Rok vydání: | 2015 |
| Předmět: |
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Molecular Protein Conformation Mutant Biophysics Intracellular Space Biology Fatty Acid-Binding Proteins Biochemistry Polymorphism Single Nucleotide Fatty acid-binding protein Cell Line Mice Structural Biology Genetics Fatty acid binding protein Subcellular localizatoin Animals Humans Polymorphism Muscle Skeletal Molecular Biology Protein secondary structure chemistry.chemical_classification Single-nucleotide polymorphism Protein Stability Fatty Acids Fatty acid Cell Biology Metabolism Subcellular localization Protein Transport chemistry Mutation Fatty Acid Binding Protein 3 Intracellular |
| Zdroj: | FEBS letters. 589(18) |
| ISSN: | 1873-3468 |
| Popis: | Fatty acid-binding proteins (FABP) play a crucial role in intracellular fatty acid transportation and metabolism. In this study, we investigate the effects of the FABP3 Asp3Gly (D3G) polymorphism on protein structure and function. Although the mutation did not alter protein secondary structure or the ability to bind 1-anilinonaphthalene-8-sulfonic acid and palmitate, the intracellular stability of the D3G mutant was significantly decreased. Immunocytochemical analysis reveals that the mutation alters FABP3 subcellular localization. Our results suggest that the D3G polymorphism may impact energy metabolism and physiological functions. |
| Databáze: | OpenAIRE |
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