Lyme Neuroborreliosis in 2 Horses
| Autor: | Kimberley J. Olsen, Sunlian Feng, Jocelyn M. Johnston, J. J. Bertone, Stephen W. Barthold, Denise M. Imai, B. C. Barr, Emir Hodzic, B. Daft |
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| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial Male Pathology medicine.medical_specialty law.invention Lyme disease Species Specificity law medicine Animals Lyme Neuroborreliosis Horses Borrelia burgdorferi Polymerase chain reaction Antigens Bacterial General Veterinary biology Goats Muscles Leptomeninges Sequence Analysis DNA bacterial infections and mycoses medicine.disease biology.organism_classification Antibodies Bacterial Bacterial Typing Techniques LYME Spinal Cord Genes Bacterial Female Horse Diseases Rabbits Neuroborreliosis Meningitis Joint Capsule |
| Zdroj: | Veterinary Pathology. 48:1151-1157 |
| ISSN: | 1544-2217 0300-9858 |
| DOI: | 10.1177/0300985811398246 |
| Popis: | Lyme neuroborreliosis—characterized as chronic, necrosuppurative to nonsuppurative, perivascular to diffuse meningoradiculoneuritis—was diagnosed in 2 horses with progressive neurologic disease. In 1 horse, Borrelia burgdorferi sensu stricto was identified by polymerase chain reaction amplification of B burgdorferi sensu stricto–specific gene targets ( ospA, ospC, flaB, dbpA, arp). Highest spirochetal burdens were in tissues with inflammation, including spinal cord, muscle, and joint capsule. Sequence analysis of ospA, ospC, and flaB revealed 99.9% sequence identity to the respective genes in B burgdorferi strain 297, an isolate from a human case of neuroborreliosis. In both horses, spirochetes were visualized in affected tissues with Steiner silver impregnation and by immunohistochemistry, predominantly within the dense collagenous tissue of the dura mater and leptomeninges. |
| Databáze: | OpenAIRE |
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