A cocktail of thermally stable, chemically synthesized capture agents for the efficient detection of anti-gp41 antibodies from human sera
| Autor: | Jocelyn T. Kim, Kaycie Deyle, Jessica A. Pfeilsticker, Blake Farrow, Connie L. Hsueh, Aiko Umeda, James R. Heath, Bert T. Lai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
lcsh:Medicine
Peptide Enzyme-Linked Immunosorbent Assay HIV Infections Biology HIV Antibodies Signal-To-Noise Ratio Gp41 Epitope law.invention Epitopes Immune system Antigen law HIV Seropositivity Humans Amino Acid Sequence lcsh:Science chemistry.chemical_classification Multidisciplinary Molecular Structure Protein Stability lcsh:R Temperature virus diseases Molecular biology HIV Envelope Protein gp41 Antibodies Anti-Idiotypic chemistry Polyclonal antibodies Recombinant DNA biology.protein Click Chemistry lcsh:Q Antibody Peptides Protein Binding Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 10, p e76224 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | We report on a method to improve in vitro diagnostic assays that detect immune response, with specific application to HIV-1. The inherent polyclonal diversity of the humoral immune response was addressed by using sequential in situ click chemistry to develop a cocktail of peptide-based capture agents, the components of which were raised against different, representative anti-HIV antibodies that bind to a conserved epitope of the HIV-1 envelope protein gp41. The cocktail was used to detect anti-HIV-1 antibodies from a panel of sera collected from HIV-positive patients, with improved signal-to-noise ratio relative to the gold standard commercial recombinant protein antigen. The capture agents were stable when stored as a powder for two months at temperatures close to 60(o)C. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|